Sequence requirements for the activity of membrane-active peptides
被引:14
作者:
Werkmeister, JA
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机构:CSIRO Hlth Sci & Nutr, Parkville, Vic 3052, Australia
Werkmeister, JA
Hewish, DR
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机构:CSIRO Hlth Sci & Nutr, Parkville, Vic 3052, Australia
Hewish, DR
Kirkpatrick, A
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机构:CSIRO Hlth Sci & Nutr, Parkville, Vic 3052, Australia
Kirkpatrick, A
Rivett, DE
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机构:CSIRO Hlth Sci & Nutr, Parkville, Vic 3052, Australia
Rivett, DE
机构:
[1] CSIRO Hlth Sci & Nutr, Parkville, Vic 3052, Australia
[2] CSIRO Mol Sci, Parkville Lab, Parkville, Vic 3052, Australia
来源:
JOURNAL OF PEPTIDE RESEARCH
|
2002年
/
60卷
/
04期
关键词:
activity;
analogues;
melittin;
sequence;
D O I:
10.1034/j.1399-3011.2002.21011.x
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
Synthetic peptides were constructed with the sequence of the first 20 residues of melittin and terminating with a range of different amino acid amides. These were found to have haemolytic and cytolytic activity similar to that of melittin, provided that certain charge constraints were observed. The nature of the 21st residue was not critical except when the residue introduced a negative charge. The presence of at least two positive charges in the molecule was found to be essential for activity. One of these charges could be the amino-terminal amine. Peptides could be inactivated by the addition of a non-acidic presequence which was acetylated at the N-terminus. Introducing a protease cleavable sequence into an N-terminal extension of the peptides produced analogues with low haemolytic activity that could be activated by proteolytic action. A peptide with extra positive charges introduced on the hydrophilic face of the helix possessed a haemolytic activity that was greater than that of melittin.
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页码:232 / 238
页数:7
相关论文
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[1]
BLONDELLE S E, 1991, Peptide Research, V4, P12
机构:
TORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USATORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USA
BLONDELLE, SE
HOUGHTEN, RA
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机构:
TORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USATORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USA
机构:
TORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USATORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USA
BLONDELLE, SE
HOUGHTEN, RA
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机构:
TORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USATORREY PINES INST MOLEC STUDIES, 3550 GEN ATOM COURT, SAN DIEGO, CA 92121 USA