Practical Applications of Asymmetrical Flow Field-Flow Fractionation (AF4): A Review

被引:0
作者
Leeman, Mats [1 ]
Storm, Matilda Ulmius [1 ]
Nilsson, Lars [2 ]
机构
[1] SOLVE Res & Consultancy AB, Lund, Sweden
[2] Lund Univ, Dept Food Technol Engn & Nutr, S-22100 Lund, Sweden
关键词
SIZE-EXCLUSION CHROMATOGRAPHY; ETHYLHYDROXYETHYL CELLULOSE; MOLECULAR-MASS; BETA-GLUCAN; LIPOSOMES; SEPARATION; MACROMOLECULES; DEGRADATION; BEHAVIOR;
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Characterization of macromolecules and colloids is an area of considerable interest. Asymmetrical flow field-flow fractionation (AF4) has become a well-established method, but many potential users possess limited knowledge of its capabilities, or how it can provide additional information or serve as validation to the traditional analytical techniques. This review article highlights several practical applications where AF4 should be given special consideration, and discusses benefits and drawbacks of the different methods.
引用
收藏
页码:642 / 651
页数:10
相关论文
共 54 条
[1]   LIPOSOME DISPOSITION INVIVO .3. DOSE AND VESICLE-SIZE EFFECTS [J].
ABRA, RM ;
HUNT, CA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 666 (03) :493-503
[2]   Size and structure characterization of ethylhydroxyethyl cellulose by the combination of field-flow fractionation with other techniques.: Investigation of ultralarge components [J].
Andersson, M ;
Wittgren, B ;
Schagerlöf, H ;
Momcilovic, D ;
Wahlund, KG .
BIOMACROMOLECULES, 2004, 5 (01) :97-105
[3]   Ultrahigh molar mass component detected in ethylhydroxyethyl cellulose by asymmetrical flow field-flow fractionation coupled to multiangle light scattering [J].
Andersson, M ;
Wittgren, B ;
Wahlund, KG .
ANALYTICAL CHEMISTRY, 2001, 73 (20) :4852-4861
[4]  
[Anonymous], THESIS
[5]   Rationalizing Nanomaterial Sizes Measured by Atomic Force Microscopy, Flow Field-Flow Fractionation, and Dynamic Light Scattering: Sample Preparation, Polydispersity, and Particle Structure [J].
Baalousha, M. ;
Lead, J. R. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2012, 46 (11) :6134-6142
[6]  
Caldwell K.D., 1991, POLYM ANAL FIELD FLO
[7]   Separation and Characterization of Protein Aggregates and Particles by Field Flow Fractionation [J].
Cao, Shawn ;
Pollastrini, Joey ;
Jiang, Yijia .
CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2009, 10 (04) :382-390
[8]   Characterization of Starch by Size-Exclusion Chromatography: The Limitations Imposed by Shear Scission [J].
Cave, Richard A. ;
Seabrook, Shane A. ;
Gidley, Michael J. ;
Gilbert, Robert G. .
BIOMACROMOLECULES, 2009, 10 (08) :2245-2253
[9]  
Frantzen C. B., 2003, AAPS PHARMSCITECH, V4, P62
[10]  
Frater DJ, 1997, J POLYM SCI POL PHYS, V35, P141, DOI 10.1002/(SICI)1099-0488(19970115)35:1<141::AID-POLB12>3.0.CO