Diagnostic value of the isotype-specific ELISA for the detection of antibodies against swine vesicular disease virus (SVDV)

被引：0

作者：

Niedbalski, W ^{[1
]}

机构：

[1] Natl Inst Vet Res, Dept Foot & Mouth Dis, PL-98220 Zdunska Wola, Poland

来源：

BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2002年 / 46卷 / 01期

关键词：

pig; SVD; isotype-specific ELISA; singleton reactors;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A trapping-indirect ELISA was used to determine the isotype of the antibody against SVDV in pigs experimentally infected with SVDVPOL/73. The first antibodies were detectable as early as 4 days after experimental infection. Up to the 9th day, demonstrable antibodies were exclusively of the IgM class. At this day the IgG developed and remained as a plateaux level up to the 28th d.p.i. The similar results were obtained in pigs immunized with experimental vaccine prepared from SVDV antigen. The use of trapping-indirect ELISA allowed to significantly reduce the number of non-specific positive results (singleton reactors) obtained by virus neutralization test and MAC-ELISA method.

引用

页码：37 / 44

页数：8

共 18 条

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