Investigation on electrochemical behavior of the Langmuir-Blodgett film of bilirubin

The electrochemical behavior of mixed Langmuir-Blodgett (LB) film of bilirubin and phosphatidyl ethanolamine on glassy carbon electrode were investigated with cyclic voltammetry and differential pulse voltammetry. The results prove that bilirubin Langmuir-Blodgett film has a cathodic peak of + 420mV in 0.2mol.L-1 HAc-NaAc-50% EtOH (pH = 5.4) solution. The peak current increases with the layer number of bilirubin LB film in the range of 1 to 12 layers. The result of the electrochemical behavior of bilirubin in ordered LB film was also compared with that in cast film and in aqueous solution at glassy carbon electrode. We found that bilirubin has the similar electrochemical behavior in LB film to in cast film, but different from that in aqueous solution.