Gene Cloning and Gene Expression of Hsp90 from Meloidogyne incognita under the Temperature and Heavy Metal Stress

The full length cDNA (2353 bp) of Hsp90 from Meloidogyne incognita was isolated through the rapid amplification of cDNA ends (RACE) method. The homology analysis revealed M. incognita Hsp90 amino acid sequence shared high similarity with Hsp90s of other eukaryotes. Based on QRT-PCR analysis, it suggested that the relative expression level of M. incognita juvenile (J2) improved subjected to heat shock, cold shock or heavy metal stress-a higher Mi-Hsp90 expression level compared with its CK (25 degrees C) subjected to stress at 39 degrees C and still higher than that subjected to cold shock or heavy metal stress-32.47 times as much as its CK's level at 6 h after heat shock and 10.06 times as much as its CK's level at a peak, 1 h after cold shock (4 degrees C), while it was 4.01 times as much as its CK's level at a peak, 24 h after heavy metal stress. The relative Mi-Hsp90 expression level subjected to heavy metal stress was lowest, but it was higher compared with CK whatever stressor there might be. That indicates J2 Mi-Hsp90 different expressions equally when exposed to heat shock, cold shock or heavy metal stress, and will serve communications between plant parasitic nematodes and their environment. (C) 2014 Friends Science Publishers