Oligonucleotides-Decorated-Poly(N-vinyl pyrrolidone) Nanogels for Gene Delivery

被引：29

作者：

Dispenza, Clelia ^{[1
,2
]}

Adamo, Giorgia ^{[3
]}

Sabatino, Maria Antonietta ^{[1
]}

Grimaldi, Natascia ^{[1
]}

Bulone, Donatella ^{[2
]}

Bondi, Maria Luisa ^{[4
]}

Rigogliuso, Salvatrice ^{[3
]}

Ghersi, Giulio ^{[3
]}

机构：

[1] Univ Palermo, Dipartimento Ingn Chim, I-90128 Palermo, Italy

[2] CNR Ist Biofis IBF UOS Palermo, I-90146 Palermo, Italy

[3] Univ Palermo, Dipartimento Sci & Tecnol Biol Chim & Farmaceut S, I-90128 Palermo, Italy

[4] CNR Ist Studio Mat Nanostrutturati ISMN UOS Paler, I-90146 Palermo, Italy

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2014年 / 131卷 / 02期

关键词：

biomedical applications; gels; irradiation; DNA; NETWORKS; VECTORS; SYSTEMS;

D O I：

10.1002/app.39774

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Pulsed electron-beam irradiation of a semi-dilute poly(N-vinyl pyrrolidone) (PVP) aqueous solution in the presence of acrylic acid has led to a carboxyl functionalized nanogel system. Nanoparticles hydrodynamic size and surface charge density, in water and as a function of pH, were investigated by dynamic light scattering and laser doppler velocimetry, respectively. Nanogels (NGs) were proved not to be cytotoxic at the cellular level. Indeed, they rapidly bypass the cellular membrane to accumulate in specific cell portions of the cytoplasm, in the perinuclear area. The availability of pendant carboxyl groups on the crosslinked PVP NGs core prompted us to attempt their decoration with a single strand oligonucleotide, which holds a terminal amino group. The recognition ability of the attached single helix of its complementary strand was investigated. (C) 2013 Wiley Periodicals, Inc.

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页数：8

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