Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

被引：1
作者：
del Rosario Espinoza-Mellado, Maria [1 ]
Oliver Lopez-Villegas, Edgar [2 ]
Arteaga-Garibay, Ramon I. [3 ]
Giono-Cerezo, Silvia [1 ]
机构：
[1] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Lab Bacteriol Med, Mexico City, DF, Mexico
[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Invest, Mexico City, DF, Mexico
[3] Inst Nacl Invest Forestales & Agr, Ctr Nacl Recursos Genet, Lab Recursos Genet Microbianos, Tepatitlan, Jalisco, Mexico
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2013年 / 108卷 / 08期
关键词：
Haemophilus influenzae; cell vacuolation; cytotoxicity assays; ESCHERICHIA-COLI; HEMOLYSIN; AUTOPHAGY; CYTOTOXIN;
D O I：
10.1590/0074-0276130716
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a "vacuolating factor" in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae.
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页码：1074 / +
页数：5
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