Telomerase, hTERT and splice variants in patients with myelodysplastic syndromes

被引:21
作者
Dong, Wen [1 ]
Qian, Yaqin [2 ,3 ,4 ]
Yang, Lili [2 ,3 ,4 ]
机构
[1] Tianjin Hongqiao Hosp, Dept Orthopaed Surg, Tianjin, Peoples R China
[2] Tianjin Med Univ, Tianjin Canc Inst & Hosp, Dept Immunol, Tianjin 300060, Peoples R China
[3] Natl Clin Res Ctr Canc, Beijing, Peoples R China
[4] Key Lab Canc Immunol & Biotherapy, Tianjin, Peoples R China
关键词
Myelodysplastic syndrome; Telomere; Senescence; Homeostasis; Splice variant; REPEAT AMPLIFICATION PROTOCOL; UNITED-STATES; CANCER-CELLS; QUANTIFICATION; EXPRESSION; PCR;
D O I
10.1016/j.leukres.2014.04.008
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Telomeres are specialized structures maintaining chromosome integrity during cellular division and preventing from premature senescence and apoptosis. The rate-limiting component of telomerase is human telomerase reverse transcriptase (hTERT), for which multiple transcripts exist. The aim of this work was to characterize hTERT splice variants in MDS and its relation to telomerase activity, telomere length and hTERT expression. The telomere length in PBMCs of patients with MDS cases was significantly shorter compared to controls (n = 30, p = 0.002). MDS patients had significantly higher basal telomerase activity (p = 0.022) and higher total hTERT (p = 0.007), alpha+beta + hTERT variant (p = 0.016) and alpha+beta - hTERT variant expression than control. The ratio of alpha+beta - transcript to alpha+beta + transcript was significantly increased in cases (p = 0.039). This study provided a detailed insight into the hTERT transcript pattern in MDS while correlation analysis showed that only telomerase activity was significantly correlated with total hTERT expression in MDS. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:830 / 835
页数:6
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