Accumulation of catechins in tea in relation to accumulation of mRNA from genes involved in catechin biosynthesis

被引：47

作者：

Eungwanichayapant, P. D. ^{[1
]}

Popluechai, S. ^{[1
]}

机构：

[1] Mae Fah Luang Univ, Sch Sci, Chiang Rai 57100, Thailand

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2009年 / 47卷 / 02期

关键词：

Tea; Camellia sinensis; Catechins; RT-PCR; HPLC; CAMELLIA-SINENSIS; GREEN TEA; EXPRESSION; HPLC;

D O I：

10.1016/j.plaphy.2008.11.002

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Catechins are a group of polyphenols found in tea (Camellia sinensis var. sinensis) at high levels. They are beneficial for health. From the study on accumulation of catechins in shoots and mature leaves of a tea cultivar, Oolong No. 17, using high-performance liquid chromatography (HPLC), it was found that the amounts of most catechins in the shoots were higher than those in the mature leaves, with an exception of catechins gallate (CG) that was found in trace amounts in both the shoots and mature leaves. mRNA accumulation of genes involved in catechin synthesis was studied using reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that the mRNA accumulation of the genes were higher in the shoots than in the mature leaves. These genes included genes of phenylalanine ammonialyase 1 (PAL1; EC 4.3.1.5), chalcone synthase (CHS; EC 2.3.1.74), dihydroflavonol 4-reductase (DFR; EC 1.1.1.219), leucoanthocyanidin reductase (LCR; EC 1.17.1.3), and flavanone 3-hydroxylase (F3H; EC 1.14.11.9). (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：94 / 97

页数：4

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