Dual Functionalized Injectable Hybrid Extracellular Matrix Hydrogel for Burn Wounds

被引：28

作者：

Bankoti, Kamakshi ^{[1
]}

Rameshbabu, Arun Prabhu ^{[1
]}

Datta, Sayanti ^{[1
]}

Goswami, Piyali ^{[2
]}

Roy, Madhurima ^{[2
]}

Das, Dipankar ^{[3
]}

Ghosh, Sudip Kumar ^{[2
]}

Das, Amit Kumar ^{[2
]}

Mitra, Analava ^{[4
]}

Pal, Sagar ^{[3
]}

Maulik, Dhrubajyoti ^{[5
]}

Su, Bo ^{[6
]}

Ghosh, Paulomi ^{[7
]}

Basu, Bikramajit ^{[8
]}

Dhara, Santanu ^{[1
]}

机构：

[1] Indian Inst Technol Kharagpur, Sch Med Sci & Technol, Biomat & Tissue Engn Lab, Kharagpur 721302, W Bengal, India

[2] Indian Inst Technol Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India

[3] Indian Sch Mines, Dept Appl Chem, Polymer Chem Lab, Indian Inst Technol, Dhanbad 826004, Bihar, India

[4] Indian Inst Technol Kharagpur, Sch Med Sci & Technol, Nat Prod Res Lab, Kharagpur 721302, W Bengal, India

[5] Bankura Sammilani Med Coll, Dept Surg, Bankura 722102, India

[6] Univ Bristol, Bristol Dent Sch, Bristol BS1 2LY, Avon, England

[7] CSIR Indian Inst Chem Biol CSIR IICB, Struct Biol & Bioinformat, Kolkata 700032, India

[8] Indian Inst Sci, Mat Res Ctr, Bangalore 560012, Karnataka, India

来源：

BIOMACROMOLECULES | 2021年 / 22卷 / 02期

基金：

英国医学研究理事会;

关键词：

Crosslinking - Biodegradation - Escherichia coli - Biodegradability - Cell culture;

D O I：

10.1021/acs.biomac.0c01400

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Low strength and rapid biodegradability of acellular dermal matrix (ADM) restrict its wider clinical application as a rapid cell delivery platform in situ for management of burn wounds. Herein, the extracted ADM was modified by a dual cross-linking approach with ionic crosslinking using chitosan and covalent crosslinking using an iodine-modified 2,5-dihydro-2,5-dimethoxy-furan cross-linker, termed as CsADM-Cl. In addition, inherent growth factors and cytokines were found to be preserved in CsADM-Cl, irrespective of ionic/covalent crosslinking. CsADM-Cl demonstrated improvement in post crosslinking stiffness with a decreased biodegradation rate. This hybrid crosslinked hydrogel supported adhesion, proliferation, and migration of human foreskin-derived fibroblasts and keratinocytes. Also, the angiogenic potential of CsADM-Cl was manifested by chick chorioallantoic membrane assay. CsADM-Cl showed excellent antibacterial activity against Escherichia coli and Staphylococcus aureus. Moreover, CsADM-Cl treated full thickness bum wounds and demonstrated rapid healing marked with superior angiogenesis, well-defined dermal-epidermal junctions, mature basket weave collagen deposition, and development of more pronounced secondary appendages. Altogether, the bioactive CsADM-Cl hydrogel established significant clinical potential to support wound healing as an apt injectable antibacterial matrix to encounter unmet challenges concerning critical burn wounds.

引用

页码：514 / 533

页数：20

共 75 条

[1] Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells [J].