iPSC-Derived Retina Transplants Improve Vision in rdl End-Stage Retinal-Degeneration Mice

被引：130

作者：

Mandai, Michiko ^{[1
]}

Fujii, Momo ^{[1
]}

Hashiguchi, Tomoyo ^{[1
]}

Sunagawa, Genshiro A. ^{[1
]}

Ito, Shin-ichiro ^{[1
]}

Sun, Jianan ^{[1
]}

Kaneko, Jun ^{[1
]}

Sho, Junki ^{[1
]}

Yamada, Chikako ^{[1
]}

Takahashi, Masayo ^{[1
]}

机构：

[1] RIKEN Ctr Dev Biol, Lab Retinal Regenerat, Chuo Ku, 2-2-3 Minatojima Minamimachi, Kobe, Hyogo 6500047, Japan

来源：

STEM CELL REPORTS | 2017年 / 8卷 / 01期

关键词：

GANGLION-CELLS; RESTORATION; CACNA1S; DONOR; RODS;

D O I：

10.1016/j.stemcr.2016.12.008

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Recent success in functional recovery by photoreceptor precursor transplantation in dysfunctional retina has led to an increased interest in using embryonic stem cell (ESC) or induced pluripotent stem cell (iPSC)-derived retinal progenitors to treat retinal degeneration. However, cell-based therapies for end-stage degenerative retinas that have lost the outer nuclear layer (ONL) are still a big challenge. In the present study, by transplanting mouse iPSC-derived retinal tissue (miPSC retina) in the end-stage retinal-degeneration model (rd1), we visualized the direct contact between host bipolar cell terminals and the presynaptic terminal of graft photoreceptors by gene labeling, showed light-responsive behaviors in transplanted rd1 mice, and recorded responses from the host retina with transplants by ex vivo micro-electroretinography and ganglion cell recordings using a multiple-electrode array system. Our data provides a proof of concept for transplanting ESC/iPSC retinas to restore vision in end-stage retinal degeneration.

引用

收藏

页码：69 / 83

页数：15

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