Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from the Endometrium and Endometriosis Lesions of Women with Malformations of the Internal Reproductive Organs

被引：5

作者：

Savilova, A. M. ^{[1
]}

Farkhat, K. N. ^{[1
]}

Yushina, M. N. ^{[1
]}

Rudimova, Yu. V. ^{[1
]}

Makiyan, Z. N. ^{[1
]}

Adamyan, L. V. ^{[1
]}

机构：

[1] Minist Hlth Russian Federat, VI Kulakov Res Ctr Obstet Gynecol & Perinatol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2017年 / 162卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

endometriosis; endometrium; multipotent mesenchymal stromal cells; immunophenotype; malformations of the internal reproductive organs; STEM-CELLS; EXPRESSION; STAGE; CAPACITY;

D O I：

10.1007/s10517-017-3656-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We isolated and characterized cell cultures from eutopic endometrium and endometriotic lesions of women with malformations of the internal reproductive organs. The cells had fibroblast-like shape and intensively expressed CD90, CD73, CD105, CD44, CD146, and CD117 and were capable of induced adipogenic and osteogenic differentiation in vitro. The obtained cultures exhibited properties of multipotent mesenchymal stromal cells; at the same time, they demonstrated in vitro immunophenotypic differences from cell cultures of eutopic and ectopic endometrium of women without developmental abnormalities, which suggests their functional difference. The cells from eutopic endometrium and from ectopic endometriotic lesions can be used as the model for studying of the etiology and pathogenesis of endometriosis and for testing new drugs for this specific group of patients. Markers CD90 and CD117 were identified as promising molecules for the development of minimally invasive diagnostics of endometriosis based on cell cultures from eutopic endometrium.

引用

页码：539 / 544

页数：6

共 22 条

[1]

Adamyan LV, 2015, PROBL REPROD, p[21, 8], DOI 10.17116/repro20152158-16

[2]

Adamyan LV, 1998, GENETIC ASPECTS GYNE

[3]

Adamyan LV, 1998, MALFORMATIONS UTERUS

[4] The prevalence of congenital uterine anomalies in unselected and high-risk populations: a systematic review [J].

Chan, Y. Y. ;

Jayaprakasan, K. ;

Zamora, J. ;

Thornton, J. G. ;

Raine-Fenning, N. ;

Coomarasamy, A. .

HUMAN REPRODUCTION UPDATE, 2011, 17 (06) :761-771

[5] Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].

Dominici, M. ;

Le Blanc, K. ;

Mueller, I. ;

Slaper-Cortenbach, I. ;

Marini, F. C. ;

Krause, D. S. ;

Deans, R. J. ;

Keating, A. ;

Prockop, D. J. ;

Horwitz, E. M. .

CYTOTHERAPY, 2006, 8 (04) :315-317

[6]

[Фархат К.Н. Farkhat K.N.], 2016, [Проблемы репродукции, Problemy reproduktsii], V22, P20

[7] Endometrial stem/progenitor cells: the first 10 years [J].

Gargett, Caroline E. ;

Schwab, Kjiana E. ;

Deane, James A. .

HUMAN REPRODUCTION UPDATE, 2016, 22 (02) :137-163

[8] Endometrial reconstruction from stem cells [J].

Gargett, Caroline E. ;

Ye, Louie .

FERTILITY AND STERILITY, 2012, 98 (01)

[9] Menstrual endometrial cells from women with endometriosis demonstrate increased adherence to peritoneal cells and increased expression of CD44 splice variants [J].

Griffith, Jason S. ;

Liu, Ya-Guang ;

Tekmal, Rajeshwar R. ;

Binkley, Peter A. ;

Holden, Alan E. C. ;

Schenker, Robert S. .

FERTILITY AND STERILITY, 2010, 93 (06) :1745-1749

[10] Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures [J].

Jazedje, Tatiana ;

Perin, Paulo M. ;

Czeresnia, Carlos E. ;

Maluf, Mariangela ;

Halpern, Silvio ;

Secco, Mariane ;

Bueno, Daniela F. ;

Vieira, Natassia M. ;

Zucconi, Eder ;

Zatz, Mayana .

JOURNAL OF TRANSLATIONAL MEDICINE, 2009, 7

← 1 2 3 →