Cross-talk Calibrating Based on Correlation Analysis in High-throughput Genome Sequencing

被引：0

作者：

Ye, Bing-gang ^{[1
]}

Wu, Xiao-ming ^{[1
]}

Li Jing-xiang ^{[2
]}

Yan, Zhou ^{[2
]}

Wang De-peng ^{[2
]}

机构：

[1] South China Univ Technol, Sch Biosci & Bioengn, Guangzhou, Guangdong, Peoples R China

[2] Shenzhen BGI, Shenzhen, Peoples R China

来源：

2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010) | 2010年

关键词：

High-throughput; Sequencing; Cross-talk; correlation analysis; MATRIX; SYSTEM;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The images, in high-throughput genome sequencing of sequencing by synthesize, consist of A, C, G, T base fluorescence images, and the fluorophore emission spectra partially overlap, which causes cross-talk problem. This paper presents a method of correlation analysis, construct the calibration matrix to eliminate interference of the fluorophore. The experiment result shows the method provided in this article is effective to correct cross-talk influence, and get the deconvolution of the clusters signal.

引用

页数：4

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