Cross-talk Calibrating Based on Correlation Analysis in High-throughput Genome Sequencing

被引：0
作者：
Ye, Bing-gang [1 ]
Wu, Xiao-ming [1 ]
Li Jing-xiang [2 ]
Yan, Zhou [2 ]
Wang De-peng [2 ]
机构：
[1] South China Univ Technol, Sch Biosci & Bioengn, Guangzhou, Guangdong, Peoples R China
[2] Shenzhen BGI, Shenzhen, Peoples R China
来源：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010) | 2010年
关键词：
High-throughput; Sequencing; Cross-talk; correlation analysis; MATRIX; SYSTEM;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The images, in high-throughput genome sequencing of sequencing by synthesize, consist of A, C, G, T base fluorescence images, and the fluorophore emission spectra partially overlap, which causes cross-talk problem. This paper presents a method of correlation analysis, construct the calibration matrix to eliminate interference of the fluorophore. The experiment result shows the method provided in this article is effective to correct cross-talk influence, and get the deconvolution of the clusters signal.
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页数：4
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