Electrochemical studies of o-tilidine as a hydrogen donor substrate for peroxidase and its application in enzyme immunoassay

被引：14

作者：

Jiao, K ^{[1
]}

Sun, W ^{[1
]}

机构：

[1] Oingdao Inst Chem Technol, Dept Appl Chem, Shandong 266042, Peoples R China

来源：

MICROCHEMICAL JOURNAL | 2002年 / 72卷 / 02期

基金：

中国国家自然科学基金;

关键词：

o-tilidine; horseradish peroxidase; enzyme immunoassay; cucumber mosaic virus; voltammetry;

D O I：

10.1016/S0026-265X(01)00116-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive electrochemical method is described for assay of horseradish peroxidase (HRP) using o-tilidine as substrate. Under the optimal conditions, the detection limit for HRP is 2.5 mU/l and the calibration range is from 5.0 to 1000 mU/l. The relative standard deviation of 11 measurements is 6.3% for 10.0 mU/l HRP. This new electrochemical system was further combined with an indirect enzyme immunoassay using direct antigen-coating format for the detection of cucumber mosaic virus (CMV). The results show an improved sensitivity over the traditional o-phenylenediamine (OPD) spectrophotometric enzyme-linked immunosorbent assay (ELISA) method. Using this technique, a minimum detectable level of 2.0 ng/ml of purified CMV and 1:12500 dilution of CMV in infected leaf extractions can be achieved. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 130

页数：8