Development, Characterization Optimization, and Assessment of Curcumin-Loaded Bioactive Self-Nanoemulsifying Formulations and Their Inhibitory Effects on Human Breast Cancer MCF-7 Cells

被引:23
作者
Kazi, Mohsin [1 ]
A. Nasr, Fahd [2 ]
Noman, Omar [2 ]
Alharbi, Abdulrahman [1 ]
Alqahtani, Mohammed S. [1 ]
Alanazi, Fars K. [1 ]
机构
[1] King Saud Univ, Coll Pharm, Dept Pharmaceut, Riyadh 11451, Saudi Arabia
[2] King Saud Univ, Med Aromat & Poisonous Plants Res Ctr, Coll Pharm, Riyadh 11451, Saudi Arabia
关键词
bioactive self-nanoemulsifying drug delivery systems (Bio-SNEDDSs); solubility improvement; curcumin; thymoquinone; antioxidant activity; drug precipitation; MTT assay; DRUG-DELIVERY SYSTEMS; LIPID-BASED FORMULATIONS; ORAL BIOAVAILABILITY ENHANCEMENT; IN-VITRO; PARTICLE-SIZE; THYMOQUINONE; PRECIPITATION; DISPERSION; DESIGN; SMEDDS;
D O I
10.3390/pharmaceutics12111107
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Curcumin (CUR) is an attractive polyphenol for its anti-inflammatory, antibacterial, antioxidant, and anticancer properties. Poor solubility in water and sensitivity against sunlight are the most challenging characteristics in the development of CUR for clinical use. The aim is to develop oral lipid-based bioactive self-nanoemulsifying drug delivery systems (Bio-SNEDDSs) for curcumin as a candidate for cancer therapy. Bio-SNEDDSs containing black seed oil, medium-chain mono- and diglycerides, and surfactants were prepared as CUR delivery vehicles. The morphology, droplet size, physical stability, encapsulation efficiency, risk of precipitation, lipid digestion, antioxidant activity, and antimicrobial activity were evaluated for the representative formulations. Finally, an MTT assay was performed on MCF-7 cells to determine the cytotoxic effect of the different formulations. The results showed lower droplet size (28.53 nm) and higher drug-loading (CUR 20 mg, thymoquinone 1.2 mg) for the representative Bio-SNEDDS (black seed oil/Imwitor 988/KolliphorEL (35/15/50) % w/w), along with a transparent appearance upon aqueous dilution. The dynamic dispersion and in-vitro lipolysis data proved that the Bio-SNEDDS was able to keep the CUR in a solubilized form in the gastrointestinal tract. From the antioxidant and antimicrobial studies, it was suggested that the Bio-SNEDDS had the highest activity for disease control. The MTT assay showed that the representative Bio-SNEDDS treatment led to a reduction of cell viability of MCF-7 cells compared to pure CUR and conventional SNEDDSs. A Bio-SNEDDS with elevated entrapment efficiency, antioxidant/antimicrobial activities, and an antiproliferative effect could be the best anticancer drug candidate for potential oral delivery.
引用
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页码:1 / 19
页数:19
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