IDENTIFICATION OF KURARINONE BY LC/MS AND INVESTIGATION OF ITS THERMAL STABILITY

被引：0

作者：

Liu, Pengfei ^{[1
,2
]}

Deng, Tiansheng ^{[1
]}

Ye, Cuiping ^{[3
]}

Qin, Zhangfeng ^{[1
]}

Hou, Xianglin ^{[1
,2
]}

Wang, Jianguo ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Coal Chem, State Key Lab Coal Convers, Taiyuan 030001, Shanxi, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

[3] Taiyuan Univ Technol, Minist Educ & Shanxi Prov, Key Lab Coal Sci & Technol, Taiyuan 030024, Shanxi, Peoples R China

来源：

JOURNAL OF THE CHILEAN CHEMICAL SOCIETY | 2009年 / 54卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Sophora flavescens Ait; Leguminosae; Flavonoids; Kurarinone; HPLC-ESI-MS; Ultrasonic extraction; Thermal stability; SOPHORA-FLAVESCENS AIT; LIQUID-CHROMATOGRAPHY; FLAVONOIDS; EXTRACTION;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A herbal ingredient kurarinone (7,2',4'-trihydroxy-8-lavandulyl-5-methoxy flavonone) was isolated from the roots of sophora flavescens Ait, by ultrasonic extraction, which is one of traditional Chinese medicine (TCM) materials. A reliable high performance liquid chromatography with mass spectrometry (HPLC-MS) method was used for determination of kurarnone. C18 column (150x4.6 mm, 5 mu m) and methanol-water mobile phase (70:30, by volume) were used in the chromatographic seperation of active components from the herb, HPLC-ESI-MS (UV, 288 nm; m/z, 437.5 [M-H]) was adopted for kurarinone identification. Kurarinone with purity of 95.5% was obtained by silica gel column followed by reverse-phase column chromatography. The thermal stability of kurarinone was investigated, which illustrates the kurarinone was stable below 25 degrees C in 48 h, but a degradation of 25.54% was observed at 80 degrees C for 2 h.

引用

页码：80 / 82

页数：3

共 11 条

[1]

Bai Y, 2004, CHEM J CHINESE U, V25, P284

[2] Plumbagin quantification in roots of Plumbago scandens L. obtained by different extraction techniques [J].