Metabolites profiling of Pulsatilla saponin D in rat by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS)

被引：69
作者：
Ouyang, Hui [1 ,2 ]
Zhou, Maofu [1 ]
Guo, Yicheng [1 ]
He, Mingzhen [1 ]
Huang, Hesong [1 ]
Ye, Xide [1 ,2 ]
Feng, Yulin [1 ]
Zhou, Xin [3 ]
Yang, Shilin [1 ,2 ]
机构：
[1] Jiangxi Univ Tradit Chinese Med, Nanchang 330006, Peoples R China
[2] Guangzhou Univ Chinese Med, Guangzhou 510405, Guangdong, Peoples R China
[3] Guizhou Normal Univ, Res Ctr Qual Control Nat Med, Guiyang 550001, Peoples R China
来源：
FITOTERAPIA | 2014年 / 96卷
关键词：
Pulsatilla saponin D; ESI Q-TOF-MS/MS; Metabolites; CHINENSIS BUNGE REGEL; PLASMA; IDENTIFICATION; GINSENOSIDES; CELLS; PHARMACOKINETICS;
D O I：
10.1016/j.fitote.2014.05.002
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Pulsatilla saponin D, an antitumor substance isolated from traditional Chinese herbal medicine Pulsatilla chinensis (Bge.) Regal, is a promising candidate for new drug development. The purpose of the present study is to establish a simple and practical strategy for the metabolite profiling of Pulsatilla saponin Din vivo. A total of 18 metabolites were identified in rat plasma, urine and feces samples based on MS and MS/MS data by using ESI-Q-TOF-MS/MS, and eight of them (M11-M18) were reported for the first time. The results indicated that deglycosylation, dehydrogenation, hydroxylation and sulfation were the major metabolic transformations of Pulsatilla saponin D in vivo. This study has improved our understanding of the metabolic fate of Pulsatilla saponin Din vivo, and the information gained from the current study is relevant to the pharmacological activity of Pulsatilla saponin D. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：152 / 158
页数：7
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