Brain-derived neurotrophic factor induces thioredoxin-1 expression through TrkB/Akt/CREB pathway in SH-SY5Y cells

被引：35

作者：

Bai, Liping ^{[1
,2
]}

Zhang, Se ^{[2
]}

Zhou, Xiaoshuang ^{[2
]}

Li, Ye ^{[2
]}

Bai, Jie ^{[2
]}

机构：

[1] Kunming Univ Sci & Technol, Fac Environm Sci & Engn, Kunming 650500, Yunnan, Peoples R China

[2] Kunming Univ Sci & Technol, Lab Mol Neurobiol, Med Fac, Kunming 650500, Yunnan, Peoples R China

来源：

BIOCHIMIE | 2019年 / 160卷

基金：

中国国家自然科学基金;

关键词：

Brain-derived neurotrophic factor; Thioredoxin-1; Tyrosine kinase B; Phosphatidylinositol; 3-kinase; cAMP response element-binding protein; OXIDATIVE STRESS; CREB; MEMORY; SIGNAL; BDNF; TRKB; INVOLVEMENT; ACTIVATION; PLASTICITY; KINASE;

D O I：

10.1016/j.biochi.2019.02.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brain-derived neurotrophic factor (BDNF) is one of the neurotrophic factors that are vital to the survival and proliferation of neuron. Thioredoxin-1 (Trx-1) is a redox regulating protein and plays various roles in regulating transcript factors and inhibiting apoptosis. It has been reported that Trx-1 is required for nerve growth factor-mediated signal transduction and neurite outgrowth, and is involved in synaptic protein expression induced by BDNF. However, the molecular mechanism on BDNF inducing Trx-1 expression has not been fully verified. The present study investigated the expression of Trx-1 after treatment with BDNF in SH-SY5Y cells. We first demonstrated that cell viability and the expression of Trx-1 were increased by BDNF in SH-SY5Y cells, which were inhibited by the tyrosine kinase B (TrkB) inhibitor, K252a, and the phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002. Moreover, BDNF increased the activity of cAMP response element-binding protein (CREB) through TrkB/PI3-K/Akt pathway. Whereas the expression of Trx-1 induced by BDNF was suppressed by CREB siRNA. Thus, our data suggest that BDNF induces the expression of Trx-1 through the TrkB/Akt/CREB pathway. (C) 2019 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

引用

页码：55 / 60

页数：6

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