Characterization of the ends and target site of a novel tetracycline resistance-encoding conjugative transposon from Enterococcus faecium 664.1H1

被引：20

作者：

Roberts, AP ^{[1
]}

Davis, IJ ^{[1
]}

Seville, L ^{[1
]}

Villedieu, A ^{[1
]}

Mullany, P ^{[1
]}

机构：

[1] Univ London, UCL, Eastman Dent Inst, Div Microbial Dis, London WC1X 8LD, England

来源：

JOURNAL OF BACTERIOLOGY | 2006年 / 188卷 / 12期

关键词：

D O I：

10.1128/JB.00129-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterococcus faecium 664.1H1 is multiply antibiotic resistant and mercury resistant. In this study, the genetic support for the tetracycline resistance of E. faecium 664.1H1 was characterized. The tet(S) gene is responsible for tetracycline resistance, and this gene is located on the chromosome of E. faecium 664.1H1, on a novel conjugative transposon. The element is transferable to Enterococcus faecalis, where it integrates into a specific site. The element was designated EfcTn1. The integrase of EfcTn1 is related to the integrase proteins found on staphylococcal pathogenicity islands. We show that the transposon is flanked by an 18-bp direct repeat, a copy of which is also present at the target site and at the joint of a circular form, and we propose a mechanism of insertion and excision.

引用

页码：4356 / 4361

页数：6

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