Identification of Nocardia Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引：31

作者：

Khot, Prasanna D. ^{[1
]}

Bird, Brian A. ^{[1
]}

Durrant, Robert J. ^{[1
,2
]}

Fisher, Mark A. ^{[1
,2
]}

机构：

[1] Inst Clin & Expt Pathol, ARUP Labs, Salt Lake City, UT USA

[2] Univ Utah, Dept Pathol, Salt Lake City, UT USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 10期

关键词：

BACTERIA; SYSTEM;

D O I：

10.1128/JCM.00780-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of Nocardia species remains challenging. By identifying 83.1% (64 of 77) and 80% (8 of 10) to the species and complex levels, respectively, and 94.3% (82 of 87) to the genus level, we show that an approach using routine sample preparation, an up-to-date commercial database minimally augmented with custom spectra, and testing at an early stage of growth is promising.

引用

页码：3366 / 3369

页数：4

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