Chitosanase activity in Bacillus thuringiensis

被引：9
|
作者：
Camarillo, RC [1 ]
Pérez, OS
Avelizapa, NGR
Ramírez, MG
Avelizapa, LIR
机构：
[1] Inst Politecn Natl, Escuela Nacl Ciencia Biol, Dept Microbiol, Mexico City 11340, DF, Mexico
[2] Inst Mexicano Petr, Programa Biotecnol, Mexico City 07730, DF, Mexico
来源：
FOLIA MICROBIOLOGICA | 2004年 / 49卷 / 01期
关键词：
D O I：
10.1007/BF02931653
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23 %) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 degreesC with shaking in a medium of pH 6.5 with 4 % colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase of B. thuringiensis.
引用
收藏
页码：94 / 96
页数：3
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