Aim. The aim of this study was to investigate the use of hypoxic sprint interval training (SIT) for the improvement of aerobic capacity. Method. Twenty-seven participants (mean +/- SD), age 21 +/- 1 yrs, body mass 72.4 +/- 9.7 kg and height 175 +/- 7 cm, completed an (V) over dotO(2peak) Incremental Exercise Test and time to exhaustion (TTE) trial (80% (V) over dotO(2peak)) pre and post SIT. Participants were randomly assigned to either, control (CONT), normoxic (NORM) or hypoxic (FiO(2): 0.15) (HYP) conditions. SIT involved 30s sprints interspersed with 4min rest. The number of sprints progressed from four to seven over six sessions separated by 1-2 days rest. Two-way mixed design ANOVA was performed to determine changes between conditions. Results. (V) over dotO(2peak) improved (P<0.05) pre to post SIT in NORM (11.2 +/- 10.8%) and HYP (10.9 +/- 6.2%), but not CONT (0.7 +/- 14.3%). TTE post SIT was significantly improved from pre SIT in NORM and HYP but not CONT (CONT=1 +/- 6, NORM=56 +/- 25, HYP=34 +/- 25%, P<0.05). Peak and recovery heart rate was lower in NORM (P<0.05) than HYP as SIT sessions progressed. SpO(2) (%) was lower in HYP (86.1 +/- 4.3%) compared to NORM (97.1 +/- 0.7%), decreasing within all HYP sessions, and increasing with SIT. Conclusion. Hypoxic and normoxic SIT caused improvement in (V) over dotO(2peak) and TTE compared to a control. Hypoxic SIT did not cause further improvements, indicating hypoxia based SIT offers no additional benefit for improvement of endurance performance.