Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

被引：17

作者：

Bobba, Kondapa Naidu ^{[1
]}

Binoy, Anupama ^{[2
]}

Koo, Seyoung ^{[3
]}

Nedungadi, Divya ^{[2
]}

Podder, Arup ^{[1
]}

Sharma, Amit ^{[3
]}

Mishra, Nandita ^{[2
]}

Kim, Jong Seung ^{[3
]}

Bhuniya, Sankarprasad ^{[1
]}

机构：

[1] Amrita Vishwa Vidyapeetham, Amrita Sch Engn, Amrita Ctr Ind Res & Innovat, Coimbatore 641112, Tamil Nadu, India

[2] Amrita Vishwa Vidyapeetham, Sch Biotechnol, Kollam 690525, Kerala, India

[3] Korea Univ, Dept Chem, Seoul 02841, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 45期

关键词：

HYDROGEN-SULFIDE;

D O I：

10.1039/c9cc01483g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore ( 2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

引用

页码：6429 / 6432

页数：4

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