A tethered bilayer lipid membrane that mimics microbial membranes

被引：34

作者：

Andersson, Jakob ^{[1
,2
]}

Fuller, Melanie A. ^{[1
,2
]}

Wood, Kathleen ^{[3
]}

Holt, Stephen A. ^{[3
]}

Koper, Ingo ^{[1
,2
]}

机构：

[1] Flinders Univ S Australia, Flinders Ctr Nanoscale Sci & Technol, Adelaide, SA, Australia

[2] Flinders Univ S Australia, Coll Sci & Engn, Adelaide, SA, Australia

[3] Australian Nucl Sci & Technol Org, Australian Ctr Neutron Scattering, Locked Bag 2001, Kirrawee Dc, NSW, Australia

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2018年 / 20卷 / 18期

关键词：

ESCHERICHIA-COLI; OUTER-MEMBRANE; POLYMYXIN-B; BACTERIAL LIPOPOLYSACCHARIDES; RESISTANCE; PROTEINS; PHOSPHOLIPIDS; REFLECTIVITY; SPECTROSCOPY; THIOLIPIDS;

D O I：

10.1039/c8cp01346b

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria. The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules. The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated. By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane. The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin. A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.

引用

页码：12958 / 12969

页数：12

共 47 条

[1] Synthesis and Characterization of Novel Anchorlipids for Tethered Bilayer Lipid Membranes [J].