RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings

被引：7

作者：

Jia, Tianran ^{[1
]}

Le, Brandon H. ^{[2
]}

机构：

[1] Cedars Sinai Med Ctr, Dept Surg, Los Angeles, CA 90048 USA

[2] Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA

来源：

BIO-PROTOCOL | 2020年 / 10卷 / 14期

基金：

美国国家卫生研究院;

关键词：

RNA Stability; RNA Degradation; Arabidopsis; RT-qPCR; Cordycepin; Primary MicroRNA Transcripts;

D O I：

10.21769/BioProtoc.3680

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive method of mRNA stability measurement through quantitative reverse transcription PCR (RT-qPCR) after inhibition of RNA transcription by cordycepin in Arabidopsis seedlings.

引用

页数：8

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