RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings

被引：7
作者：
Jia, Tianran [1 ]
Le, Brandon H. [2 ]
机构：
[1] Cedars Sinai Med Ctr, Dept Surg, Los Angeles, CA 90048 USA
[2] Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA
来源：
BIO-PROTOCOL | 2020年 / 10卷 / 14期
基金：
美国国家卫生研究院;
关键词：
RNA Stability; RNA Degradation; Arabidopsis; RT-qPCR; Cordycepin; Primary MicroRNA Transcripts;
D O I：
10.21769/BioProtoc.3680
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive method of mRNA stability measurement through quantitative reverse transcription PCR (RT-qPCR) after inhibition of RNA transcription by cordycepin in Arabidopsis seedlings.
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页数：8
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