Nuclear localization signal-dependent and -independent movements of Drosophila melanogaster dUTPase isoforms during nuclear cleavage

被引:14
|
作者
Muha, Villo [1 ]
Zagyva, Imre [1 ]
Venkei, Zsolt [2 ]
Szabad, Janos [2 ]
Vertessy, Beata G. [1 ]
机构
[1] Hungarian Acad Sci, Inst Enzymol, Lab Genome Metab & Repair, H-1518 Budapest, Hungary
[2] Univ Szeged, Fac Med, Dept Biol, Szeged, Hungary
基金
匈牙利科学研究基金会;
关键词
Nuclear cleavage; Nucleo-cytoplasmic transport; dUTPpase; Nuclear localization signal; BASE EXCISION-REPAIR; DNA; PYROPHOSPHATASE; COMPLEX; URACIL; ENZYME; PORE; IDENTIFICATION; MECHANISM; EVOLUTION;
D O I
10.1016/j.bbrc.2009.02.036
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two dUTPase isoforms (23 kDa and 21 kDa) are present in the fruitfly with the sole difference of an N-terminal extension. In Drosophila embryo, both isoforms are detected inside the nucleus. Here, we investigated the function of the N-terminal segment using eYFP-dUTPase constructs. In Schneider 2 cells, only the 23 kDa construct showed nuclear localization arguing that it may contain a nuclear localization signal (NLS). Sequence comparisons identified a lysine-rich nonapeptide with similarity to the human c-myc NLS. In Drosophila embryos during nuclear cleavages, the 23 kDa isoform showed the expected localization shifts. Contrariwise, although the 21 kDa isoform was excluded from the nuclei during interphase, it was shifted to the nucleus during prophase and forthcoming mitotic steps. The observed dynamic localization character showed strict timing to the nuclear cleavage phases and explained how both isoforms can be present within the nuclear microenvironment, although at different stages of cell cycle. (c) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:271 / 275
页数:5
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