Isolation and Characterization of Antibiotic NC0604, a New Analogue of Bleomycin

被引：17

作者：

Chen, Caixia ^{[1
]}

Si, Shuyi

He, Qiyang

Xu, Hongzhang

Lu, Min

Xie, Yunying

Wang, Yan

Chen, Ruxian

机构：

[1] Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China

来源：

JOURNAL OF ANTIBIOTICS | 2008年 / 61卷 / 12期

关键词：

NC0604; structure elucidation; biological activity; pulmonary toxicity; bleomycin;

D O I：

10.1038/ja.2008.88

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

NC0604, a new analogue of bleomycin, was isolated from fermentation broth of Streptomyces verticillus var. pingyangensis n.var. The structure of NC0604 was elucidated by spectroscopic analyses. NC0604 had the same kernel structure as bleomycin, but a different terminal amine moiety determined as amidepropyl spermidine. NC0604 exhibited antibacterial activity against a wide range of bacterial species and showed cytotoxicity in vitro against human HepG(2), KB, MCF-7, HCT116, BGC-823 and MCF-7/DOX cells with IC50 values of 1.18, 1.21, 1.41, 1.83, 2.02, 1.45,mu M, respectively. The antitumor activity of NC0604 against these cells was 3 similar to 9 times higher than that of bleomycin; and the pulmonary toxicity of NC0604 was much lower than that of bleomycin.

引用

页码：747 / 751

页数：5