Structural-functional analysis of bacteriophage T7 RNA polymerase

被引：42

作者：

Tunitskaya, VL ^{[1
]}

Kochetkov, SN ^{[1
]}

机构：

[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2002年 / 67卷 / 10期

关键词：

bacteriophage T7; DNA-dependent RNA polymerase; structural-functional topography; affinity modification; mutagenesis;

D O I：

10.1023/A:1020911223250

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review summarizes our results of the structural and functional studies of bacteriophage T7 DNA-dependent RNA polymerase (T7 RNAP). Particular features of this enzyme (the single-subunit composition, relatively low molecular weight) make it the most convenient model for investigating the physicochemical aspects of transcription. The review discusses the main properties of T7 RNAP, interaction between the enzyme and promoter, principle stages of U-transcription, and also the results of structural and functional studies by affinity modification and both random and site-directed mutagenesis techniques.

引用

页码：1124 / 1135

页数：12

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