The apple 14-3-3 protein MdGRF11 interacts with the BTB protein MdBT2 to regulate nitrate deficiency-induced anthocyanin accumulation

被引：36

作者：

Ren, Yi-Ran ^{[1
]}

Zhao, Qiang ^{[2
]}

Yang, Yu-Ying ^{[1
]}

Zhang, Tian-En ^{[1
]}

Wang, Xiao-Fei ^{[1
]}

You, Chun-Xiang ^{[1
]}

Hao, Yu-Jin ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Hort Sci & Engn, Shandong Collaborat Innovat Ctr Fruit & Vegetable, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

[2] Qingdao Agr Univ, Qingdao 266109, Shandong, Peoples R China

来源：

HORTICULTURE RESEARCH | 2021年 / 8卷 / 01期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

D O I：

10.1038/s41438-020-00457-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Nitrogen is an important factor that affects plant anthocyanin accumulation. In apple, the nitrate-responsive BTB/TAZ protein MdBT2 negatively regulates anthocyanin biosynthesis. In this study, we found that MdBT2 undergoes posttranslational modifications in response to nitrate deficiency. Yeast two-hybrid, protein pull-down, and bimolecular fluorescence complementation (BiFC) assays showed that MdBT2 interacts with MdGRF11, a 14-3-3 protein; 14-3-3 proteins compose a family of highly conserved phosphopeptide-binding proteins involved in multiple physiological and biological processes. The interaction of MdGRF11 negatively regulated the stability of the MdBT2 protein via a 26S proteasome-dependent pathway, which increased the abundance of MdMYB1 proteins to activate the expression of anthocyanin biosynthesis-related genes. Taken together, the results demonstrate the critical role of 14-3-3 proteins in the regulation of nitrate deficiency-induced anthocyanin accumulation. Our results provide a novel avenue to elucidate the mechanism underlying the induction of anthocyanin biosynthesis in response to nitrate deficiency.

引用

页数：14