Pyrosequencing Analysis of 20 Nucleotides of Internal Transcribed Spacer 2 Discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis

被引：33

作者：

Borman, Andrew M. ^{[1
]}

Linton, Christopher J.

Oliver, Debra

Palmer, Michael D.

Szekely, Adrien

Odds, Frank C. ^{[2
]}

Johnson, Elizabeth M.

机构：

[1] Hlth Protect Agcy, SW Reg Lab, Mycol Reference Lab, Bristol BS2 8EL, Avon, England

[2] Univ Aberdeen, Sch Med Sci, Aberdeen Fungal Grp, Aberdeen, Scotland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 07期

关键词：

ANTIFUNGAL SUSCEPTIBILITY; IDENTIFICATION; DNA; YEAST; EPIDEMIOLOGY; SURVEILLANCE; TECHNOLOGY; INFECTIONS; VAGINITIS; AGENTS;

D O I：

10.1128/JCM.00240-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.

引用

页码：2307 / 2310

页数：4

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