Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic® system

被引：5
作者：
Wu, Meng [1 ,2 ]
Long, Shunyou [1 ,2 ]
Frutos, Anthony G. [3 ]
Eichelberger, Maryna [4 ]
Li, Min [1 ,2 ]
Fang, Ye [3 ]
机构：
[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, High Throughput Biol Ctr, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Johns Hopkins Ion Channel Ctr, Baltimore, MD 21205 USA
[3] Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
[4] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA
来源：
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION | 2009年 / 29卷 / 3-4期
关键词：
Label free; phosphor-specific; Epic (R); 14-3-3; kinase; 14-3-3; PROTEINS; KINASE ASSAYS; FLUORESCENCE; BINDING; SITE; 14-3-3-PROTEINS; SURFACE; LIGAND; ZETA;
D O I：
10.1080/10799890903068474
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Epic(R) system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.
引用
收藏
页码：202 / 210
页数：9
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