Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic® system

被引：5

作者：

Wu, Meng ^{[1
,2
]}

Long, Shunyou ^{[1
,2
]}

Frutos, Anthony G. ^{[3
]}

Eichelberger, Maryna ^{[4
]}

Li, Min ^{[1
,2
]}

Fang, Ye ^{[3
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, High Throughput Biol Ctr, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Johns Hopkins Ion Channel Ctr, Baltimore, MD 21205 USA

[3] Corning Inc, Div Sci & Technol, Corning, NY 14831 USA

[4] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA

来源：

JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION | 2009年 / 29卷 / 3-4期

关键词：

Label free; phosphor-specific; Epic (R); 14-3-3; kinase; 14-3-3; PROTEINS; KINASE ASSAYS; FLUORESCENCE; BINDING; SITE; 14-3-3-PROTEINS; SURFACE; LIGAND; ZETA;

D O I：

10.1080/10799890903068474

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Epic(R) system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.

引用

页码：202 / 210

页数：9

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