A novel method using immuno-affinity chromatography for isolating β-conglycinin from soybean proteins

被引：9
|
作者：
You, Jinming [2 ]
Sun, Peng [1 ]
Li, Defa [1 ]
Qiao, Shiyan [1 ]
Qu, Mingren [2 ]
Wang, Zirui [2 ]
Li, Guanhong [2 ]
Pan, Ke [2 ]
机构：
[1] China Agr Univ, Natl Key Lab Anim Nutr, Beijing 100193, Peoples R China
[2] Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Peoples R China
来源：
FOOD CHEMISTRY | 2009年 / 117卷 / 02期
基金：
中国国家自然科学基金;
关键词：
beta-Conglycinin; Monoclonal antibody (Mab); Immuno-affinity chromatography; Purification; HYPERSENSITIVITY; IDENTIFICATION; FRACTIONATION; GLYCININ; ALLERGEN; MEAL;
D O I：
10.1016/j.foodchem.2009.03.098
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A monoclonal antibody (Mab) 6G(4) against soybean beta-conglycinin has been prepared using a conjugate of chicken ovalbumin and a synthetic peptide that corresponded to one of the epitope sequences of beta-conglycinin as the immunogen. An ELISA method for the quantification of beta-conglycinin has also been developed. In the present study. we report a novel method for the purification of beta-conglycinin by Mab 6G(4)-based immuno-affinity chromatography. beta-Conglycinin with a purity of 92.9% was successfully isolated from soybean proteins. Western blot assay was used to further identify its characteristics and the results demonstrated that the purified beta-conglycinin maintains its biological activities. Therefore, the Mab-based immuno-affinity chromatography is an available method for purification of beta-conglycinin. It also provides a new opportunity for future study on the mechanism of food allergy responses using high purity beta-conglycinin as the experimental material. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：371 / 374
页数：4
相关论文
共 50 条
  • [31] ISOLATION OF MAMMALIAN TYPE-C RNA VIRUS CROSS-REACTIVE ANTIGEN AND ANTIBODY BY IMMUNO-AFFINITY CHROMATOGRAPHY
    OROSZLAN, S
    BOVA, D
    GILDEN, RV
    IMMUNOCHEMISTRY, 1975, 12 (01): : 61 - 66
  • [32] The bioanalysis of the monoclonal antibody trastuzumab by high-performance liquid chromatography with fluorescence detection after immuno-affinity purification from human serum
    Damen, Carola W. N.
    Derissen, Ellen J. B.
    Schellens, Jan H. M.
    Rosing, Hilde
    Beijnen, Jos H.
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2009, 50 (05) : 861 - 866
  • [33] Determination of aflatoxin M(1) in milk and milk powder using immuno-affinity column and fluorescence measurement
    Fu, YM
    JOURNAL OF FOOD AND DRUG ANALYSIS, 1996, 4 (02) : 175 - 183
  • [34] Isolation of human proteasomes and putative proteasome-interacting proteins using a novel affinity chromatography method
    Scanlon, Thomas C.
    Gottlieb, Bruce
    Durcan, Thomas M.
    Fon, Edward A.
    Beitel, Lenore K.
    Trifiro, Mark A.
    EXPERIMENTAL CELL RESEARCH, 2009, 315 (02) : 176 - 189
  • [35] Isolating the whole complex of target proteins of FK506 using affinity resins from novel solid phases
    Takahashi, T
    Shiyama, T
    Mori, T
    Hosoya, K
    Tanaka, A
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 385 (01) : 122 - 127
  • [36] Development of an immuno-affinity column for ochratoxin analysis using an organic solvent-tolerant monoclonal antibody
    Uchigashima, Mikiko
    Yamaguchi , Yukie
    Narita, Hiroshi
    Nakajima, Masahiro
    Miyake, Shiro
    METHODS, 2012, 56 (02) : 180 - 185
  • [37] Isolating the whole complex of target proteins of FK506 using affinity resins from novel solid phases
    Teruki Takahashi
    Takaaki Shiyama
    Tomoko Mori
    Ken Hosoya
    Akito Tanaka
    Analytical and Bioanalytical Chemistry, 2006, 385 : 122 - 127
  • [38] Monolith immuno-affinity enrichment liquid chromatography tandem mass spectrometry for quantitative protein analysis of recombinant bovine somatotropin in serum
    Smits, Nathalie G. E.
    Blokland, Marco H.
    Wubs, Klaas L.
    Nessen, Merel A.
    van Ginkel, Leen A.
    Nielen, Michel W. F.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (20) : 6041 - 6050
  • [39] A SEMI-CONTINUOUS PROCESS FOR THE PRODUCTION OF HUMAN INTERFERON-ALPHA-C FROM ESCHERICHIA-COLI USING TANGENTIAL-FLOW MICROFILTRATION AND IMMUNO-AFFINITY CHROMATOGRAPHY
    VAKS, B
    MORY, Y
    PEDERSON, JU
    HOROVITZ, O
    BIOTECHNOLOGY LETTERS, 1984, 6 (10) : 621 - 626
  • [40] Development of Immunoaffinity Chromatographic Method for Isolating Glycinin (11S) from Soybean Proteins
    Hu, Shengdi
    Liu, Hong
    Qiao, Shiyan
    He, Pingli
    Ma, Xi
    Lu, Wenqing
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (18) : 4406 - 4410
12345