High-performance liquid chromatographic method for the determination of gabapentin in human plasma

被引：61
作者：
Zhu, Z [1 ]
Neirinck, L [1 ]
机构：
[1] Pharmasci, Montreal, PQ H4P 2T4, Canada
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 779卷 / 02期
关键词：
gabapentin;
D O I：
10.1016/S1570-0232(02)00399-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive high-performance liquid chromatography (HPLC) method using UV detection for the determination of gabapentin in human plasma has been developed. In this method, gabapentin was extracted from human plasma with a reversed-phase solid-phase extraction (SPE) cartridge followed by derivatization with phenylisothiocyanate. Analysis was achieved by using a HPLC system that was equipped with a UV detector. The quantitation limit of gabapentin. in human plasma was 0.03 mug/ml. The method is sensitive with excellent selectivity and reproducibility and it has been applied to a bioequivalence clinical study with great success. (C) 2002 Elsevier Science B.V. All rights reserved.
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收藏
页码：307 / 312
页数：6
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