High-performance liquid chromatographic method for the determination of gabapentin in human plasma

被引：61

作者：

Zhu, Z ^{[1
]}

Neirinck, L ^{[1
]}

机构：

[1] Pharmasci, Montreal, PQ H4P 2T4, Canada

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 779卷 / 02期

关键词：

gabapentin;

D O I：

10.1016/S1570-0232(02)00399-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive high-performance liquid chromatography (HPLC) method using UV detection for the determination of gabapentin in human plasma has been developed. In this method, gabapentin was extracted from human plasma with a reversed-phase solid-phase extraction (SPE) cartridge followed by derivatization with phenylisothiocyanate. Analysis was achieved by using a HPLC system that was equipped with a UV detector. The quantitation limit of gabapentin. in human plasma was 0.03 mug/ml. The method is sensitive with excellent selectivity and reproducibility and it has been applied to a bioequivalence clinical study with great success. (C) 2002 Elsevier Science B.V. All rights reserved.

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收藏

页码：307 / 312

页数：6

相关论文

共 14 条

[1]

*CAN PHARM ASS, 2001, COMP PHARM SPEC, P1025

[2] GABAPENTIN [J].

CHADWICK, D .

LANCET, 1994, 343 (8889) :89-91

[3] Fast isocratic high-performance liquid chromatographic assay method for the simultaneous determination of gabapentin and vigabatrin in human serum [J].

Chollet, DF ;

Goumaz, L ;

Juliano, C ;

Anderegg, G .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 746 (02) :311-314

[4] Determination of gabapentin in plasma by high-performance liquid chromatography [J].

Forrest, G ;

Sills, GJ ;

Leach, JP ;

Brodie, MJ .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1996, 681 (02) :421-425

[5]

GARCIA LL, 1995, J CHROMATOGR B, V669, P157

[6] DETERMINATION OF GABAPENTIN IN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND PRE-COLUMN LABELING FOR ULTRAVIOLET DETECTION [J].

HENGY, H ;

KOLLE, EU .

JOURNAL OF CHROMATOGRAPHY, 1985, 341 (02) :473-478

[7] DETERMINATION OF GABAPENTIN IN PLASMA AND URINE BY CAPILLARY COLUMN GAS-CHROMATOGRAPHY [J].

HOOPER, WD ;

KAVANAGH, MC ;

DICKINSON, RG .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 529 (01) :167-174

[8] Gabapentin quantification in human plasma by high-performance liquid chromatography coupled to electrospray tandem mass spectrometry. Application to bioequivalence study [J].

Ifa, DR ;

Falci, M ;

Moraes, ME ;

Bezerra, FAF ;

Moraes, MO ;

de Nucci, G .

JOURNAL OF MASS SPECTROMETRY, 2001, 36 (02) :188-194

[9] Rapid high-performance liquid chromatographic determination of serum gabapentin [J].

Jiang, QB ;

Li, SG .

JOURNAL OF CHROMATOGRAPHY B, 1999, 727 (1-2) :119-123

[10]

Kushnir MM, 1999, J ANAL TOXICOL, V23, P1