Visualizing the replication of respiratory syncytial virus in cells and in living mice

被引：105

作者：

Rameix-Welti, Marie-Anne ^{[1
,2
,3
]}

Le Goffic, Ronan ^{[1
]}

Herve, Pierre-Louis ^{[1
]}

Sourimant, Julien ^{[1
,2
]}

Remot, Aude ^{[1
]}

Riffault, Sabine ^{[1
]}

Yu, Qin ^{[4
]}

Galloux, Marie ^{[1
]}

Gault, Elyanne ^{[2
,3
]}

Eleouet, Jean-Francois ^{[1
]}

机构：

[1] INRA, Unite Virol & Immunol Mol UR892, F-78352 Jouy En Josas, France

[2] UFR Sci Sante Simone Veil UVSQ, EPIM, EA3647, F-78180 Montigny Le Bretonneux, France

[3] Hop Ambroise Pare, AP HP, Lab Microbiol, F-92100 Boulogne, France

[4] AstraZeneca R&D Boston, Infect Innovat Med Unit, Waltham, MA 02451 USA

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

IN-VIVO; VIRAL REPLICATION; GENE-EXPRESSION; INFECTION; INHIBITOR; RNA; TRANSCRIPTION; PROTEIN; CDNA; INTERFERON;

D O I：

10.1038/ncomms6104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Respiratory syncytial virus (RSV) is the most important cause of severe lower-respiratory tract disease in calves and young children, yet no human vaccine nor efficient curative treatments are available. Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome. Expression of mCherry and Luc are correlated with infection rate, allowing the monitoring of RSV multiplication in cell culture. Replication of the Luc-encoding virus in living mice can be visualized by bioluminescent imaging, bioluminescence being detected in the snout and lungs of infected mice after nasal inoculation. We propose that these recombinant viruses are convenient and valuable tools for screening of compounds active against RSV, and can be used as an extremely sensitive readout for studying effects of antiviral therapeutics in living mice.

引用

页数：10

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