Design of Ratiometric Emission Probe with Visible Light Excitation for Determination of Ca2+ in Living Cells

被引：16

作者：

Liu, Qiaoling ^{[1
,2
,3
]}

Du, Huizhi ^{[1
,2
]}

Ren, Xiaoze ^{[4
]}

Bian, Wei ^{[1
,2
,4
]}

Fan, Li ^{[1
,2
]}

Shuang, Shaomin ^{[1
,2
]}

Dong, Chuan ^{[1
,2
]}

Hu, Qin ^{[5
,6
]}

Choi, Martin M. F. ^{[5
,6
]}

机构：

[1] Shanxi Univ, Inst Environm Sci, Taiyuan 030006, Peoples R China

[2] Shanxi Univ, Sch Chem & Chem Engn, Taiyuan 030006, Peoples R China

[3] Taiyuan Normal Univ, Dept Chem, Taiyuan 030031, Peoples R China

[4] Shanxi Med Univ, Sch Basic Med Sci, Taiyuan 030001, Peoples R China

[5] Hong Kong Baptist Univ, Partner State Key Lab Environm & Biol Anal, Kowloon Tong, Hong Kong, Peoples R China

[6] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 16期

基金：

中国国家自然科学基金;

关键词：

CALCIUM; INDICATORS; FLUORESCENCE; RELEASE; SENSORS;

D O I：

10.1021/ac5022002

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca2+ determination is developed. Ca2+ can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca2+ over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca2+ is 2.25 +/- 0.47 mu M and pertinent to Ca2+ detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca2+ in living cells under confocal microscopic imaging.

引用

页码：8025 / 8030

页数：6

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