A new method for detection and quantification of heartbeat parameters in Drosophila, zebrafish, and embryonic mouse hearts

被引:228
作者
Fink, Martin [2 ,3 ]
Callol-Massot, Carles [4 ,5 ]
Chu, Angela [2 ]
Ruiz-Lozano, Pilar [1 ]
Izpisua Belmonte, Juan Carlos [5 ,6 ]
Giles, Wayne [2 ,7 ]
Bodmer, Rolf [1 ]
Ocorr, Karen [1 ]
机构
[1] Burnham Inst Med Res, Dev & Aging Program, Neurosci Aging & Stem Cell Res Ctr, La Jolla, CA 92037 USA
[2] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
[3] Univ Oxford, Dept Physiol Anat & Genet, Cardiac Electrophysiol Grp, Oxford, England
[4] Biobide, Dept Sci, San Sebastian, Gipuzkoa, Spain
[5] Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 USA
[6] Ctr Regenerat Med Barcelona, Barcelona, Spain
[7] Univ Calgary, Fac Kinesiol, Calgary, AB, Canada
基金
加拿大健康研究院; 美国国家卫生研究院;
关键词
MUTATIONS; ASSOCIATION; MODULATION; SEROTONIN; REVEALS; MODEL; GENE; TBX5; MAP;
D O I
10.2144/000113078
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The genetic basis of heart development is remarkably conserved from Drosophila to mammals, and insights from flies have greatly informed our understanding of vertebrate heart development. Recent evidence suggests that many aspects of heart function are also conserved and the genes involved in heart development also play roles in adult heart function. We have developed a Drosophda heart preparation and movement analysis algorithm that allows quantification of functional parameters. Our methodology combines high-speed optical recording of beating hearts with a robust, semi-automated analysis to accurately detect and quantify, on a beat-to-beat basis, not only heart rate but also diastolic and systolic intervals, systolic and diastolic diameters, percent fractional shortening, contraction wave velocity, and cardiac arrhythmicity. Here, we present a detailed analysis of hearts from adult Drosophila, 2-3-day-old zebrafish larva, and 8-day-old mouse embryos, indicating that our methodology is potentially applicable to an array of biological models. We detect progressive age-related changes in fly hearts as well as subtle but distinct cardiac deficits in Tbx5 heterozygote mutant zebrafish. Our methodology for quantifying cardiac function in these genetically tractable model system's should provide valuable insights into the genetics of heart function.
引用
收藏
页码:101 / +
页数:9
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