Protective effects of tryptophan-catabolizing Lactobacillus plantarum KLDS 1.0386 against dextran sodium sulfate-induced colitis in mice

被引：10
作者：
Shi, Jialu [1 ,2 ]
Du, Peng [1 ,2 ]
Xie, Qinggang [3 ]
Wang, Nana [1 ,4 ]
Li, Huizhen [1 ,2 ,5 ]
Smith, Etareri Evivie [1 ,2 ,6 ,7 ]
Li, Chun [1 ,2 ]
Liu, Fei [1 ,2 ]
Huo, Guicheng [1 ,2 ]
Li, Bailiang [1 ,2 ]
机构：
[1] Northeast Agr Univ, Key Lab Dairy Sci, Minist Educ, Harbin 150030, Peoples R China
[2] Northeast Agr Univ, Food Coll, Harbin 150030, Peoples R China
[3] Heilongjiang Feihe Dairy Co Ltd, Qiqihar 164800, Peoples R China
[4] Shandong Scents Jianyuan Biotech Co Ltd, Bingzhou 256500, Peoples R China
[5] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[6] Univ Benin, Fac Agr, Dept Food Sci & Human Nutr, Benin 300001, Nigeria
[7] Univ Benin, Fac Agr, Dept Anim Sci, Benin 300001, Nigeria
来源：
FOOD & FUNCTION | 2020年 / 11卷 / 12期
基金：
中国国家自然科学基金;
关键词：
ARYL-HYDROCARBON RECEPTOR; INFLAMMATORY-BOWEL-DISEASE; INDUCED ULCERATIVE-COLITIS; GUT MICROBIOTA; BARRIER FUNCTION; INTESTINAL MICROBIOTA; EPITHELIAL-CELLS; MUCOSA; MODEL; METABOLISM;
D O I：
10.1039/d0fo02622k
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tryptophan is an essential amino acid for the human body, whose intake is through the diet. Several studies support the theory that microbiota-derived tryptophan metabolite played a crucial role in maintaining the balance between gut microbiota and the mucosal immune system. Previously, we selected the Lactobacillus plantarum KLDS 1.0386 strain with high tryptophan-metabolic activity after the screening of 16 Lactobacillus strains. The current study aimed to assess the effects of L. plantarum KLDS 1.0386 combination with tryptophan in improving ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) and the potential mechanisms involved. Our results showed that L. plantarum KLDS 1.0386 combined with tryptophan (LAB + Trp) decreased DAI score, MPO level, and pro-inflammatory cytokine (TNF-alpha, IL-1 beta, and IL-6) concentration. It also increased anti-inflammatory cytokine (IL-10) production, tight junction proteins (claudin-1, occludin, and ZO-1), and mucin (MUC1 and MUC2) mRNA expressions. The level of indole-3-acetic acid (IAA), an important tryptophan metabolite in the liver, serum, and colon, was elevated after LAB + Trp treatment, which further upregulated aryl hydrocarbon receptor (AHR) mRNA expression to activate the IL-22/STAT3 signaling pathway. Moreover, the supplementation with LAB + Trp modulated gut microbiota composition. The present study provided novel insights that can be used to reduce the number of UC patients by employing a method utilizing tryptophan-catabolizing Lactobacillus strains.
引用
收藏
页码：10736 / 10747
页数：12
相关论文
共 70 条
  • [1] Gut Microbiota Regulation of Tryptophan Metabolism in Health and Disease
    Agus, Allison
    Planchais, Julien
    Sokol, Harry
    [J]. CELL HOST & MICROBE, 2018, 23 (06) : 716 - 724
  • [2] Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier
    Al-Sadi, Rana
    Khatib, Khaldun
    Guo, Shuhong
    Ye, Dongmei
    Youssef, Moustafa
    Ma, Thomas
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2011, 300 (06): : G1054 - G1064
  • [3] Stevioside, a diterpenoid glycoside, shows anti-inflammatory property against Dextran Sulphate Sodium-induced ulcerative colitis in mice
    Alavala, Sateesh
    Sangaraju, Rajendra
    Nalban, Nasiruddin
    Sahu, Bidya Dhar
    Jerald, Mahesh Kumar
    Kilari, Eswar Kumar
    Sistla, Ramakrishna
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 2019, 855 : 192 - 201
  • [4] Blockade of interleukin 6 trans signaling suppresses T-cell resistance against apoptosis in chronic intestinal inflammation: Evidence in Crohn disease and experimental colitis in vivo (vol 6, pg 583, 2000)
    Atreya, R.
    Mudter, J.
    Finotto, S.
    Muellberg, J.
    Jostock, T.
    Wirtz, S.
    Schuetz, M.
    Bartsch, B.
    Holtmann, M.
    Becker, C.
    Strand, D.
    Czaja, J.
    Schlaak, J. F.
    Lehr, H. A.
    Autschbach, F.
    Schuermann, G.
    Nishimoto, N.
    Yoshizaki, K.
    Ito, H.
    Kishimoto, T.
    Galle, P. R.
    Rose-John, S.
    Neurath, M. F.
    [J]. NATURE MEDICINE, 2010, 16 (11) : 1341 - 1341
  • [5] Microbiotas from Humans with Inflammatory Bowel Disease Alter the Balance of Gut Th17 and RORγt+ Regulatory T Cells and Exacerbate Colitis in Mice
    Britton, Graham J.
    Contijoch, Eduardo J.
    Mogno, Ilaria
    Vennaro, Olivia H.
    Llewellyn, Sean R.
    Ng, Ruby
    Li, Zhihua
    Mortha, Arthur
    Merad, Miriam
    Das, Anuk
    Gevers, Dirk
    McGovern, Dermot P. B.
    Singh, Namita
    Braun, Jonathan
    Jacobs, Jonathan P.
    Clemente, Jose C.
    Grinspan, Ari
    Sands, Bruce E.
    Colombel, Jean-Frederic
    Dubinsky, Marla C.
    Faith, Jeremiah J.
    [J]. IMMUNITY, 2019, 50 (01) : 212 - +
  • [6] Beneficial effect of auto-aggregating Lactobacillus crispatus on experimentally induced colitis in mice
    Castagliuolo, I
    Galeazzi, F
    Ferrari, S
    Elli, M
    Brun, P
    Cavaggioni, A
    Tormen, D
    Sturniolo, GC
    Morelli, L
    Palù, G
    [J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2005, 43 (02): : 197 - 204
  • [7] Chao L, 2017, IRAN J BASIC MED SCI, V20, P1056, DOI 10.22038/IJBMS.2017.9276
  • [8] IL-1β mediates chronic intestinal inflammation by promoting the accumulation of IL-17A secreting innate lymphoid cells and CD4+ Th17 cells
    Coccia, Margherita
    Harrison, Oliver J.
    Schiering, Chris
    Asquith, Mark J.
    Becher, Burkhard
    Powrie, Fiona
    Maloy, Kevin J.
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2012, 209 (09) : 1595 - 1609
  • [9] Cui HH, 2004, WORLD J GASTROENTERO, V10, P1521
  • [10] Akkermansia muciniphila and its role in regulating host functions
    Derrien, Muriel
    Belzer, Clara
    de Vos, Willem M.
    [J]. MICROBIAL PATHOGENESIS, 2017, 106 : 171 - 181
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