Structural Modification of Fluorescent Proteins and Their Applications in Biosensing

被引:1
作者
Li, Huiyuan [1 ]
Lei, Chunyang [1 ]
Huang, Yan [1 ]
Nie, Zhou [1 ]
机构
[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Hunan Prov Key Lab Biomacromol Chem Biol, Coll Chem & Chem Engn, Changsha 410082, Peoples R China
来源
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2020年 / 41卷 / 11期
基金
中国国家自然科学基金;
关键词
Fluorescent protein; Structural modification; Surface reconstruction; Nucleic acid mimic of fluorescent protein; Biosensing; LABEL-FREE DETECTION; IN-VIVO; VERSATILE PROBE; DI-GMP; GREEN; GFP; DELIVERY; CELLS; CHROMOPHORES; MUTANTS;
D O I
10.7503/cjcu20200405
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Since the discovery of fluorescent proteins, they have played important roles in life science, due to their genetic coding and spontaneous emission of robust fluorescence. With the development of protein engineering technology and organic synthesis, and a better understanding of the structure and function of fluorescent proteins, structural modifications and designs of fluorescent proteins were performed to give them new properties and functions, and broaden their applications in biological field such as bioimaging and biosensing. This paper focuses on the progress of the structural modification of green fluorescent protein (GFP) , as well as its mimics, including mutagenesis, barrel structure reconstruction, surface reconstruction, and nucleic acid mimics of fluorescent proteins. The representative applications of these fluorescent proteins and mimics in biosensing are also introduced.
引用
收藏
页码:2324 / 2334
页数:11
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