Determination of Escherichia coli O157: H7 in food based on chemiluminescent magnetic enzyme-linked immunoassay

被引：0

作者：

Zhu Guang-Hua ^{[1
]}

He Yan-Feng

Guo Xiao-Ying

Liu He-Ping

机构：

[1] SE Univ, Coll Publ Hlth, Nanjing 210009, Peoples R China

[2] SE Univ, Dept Biol Sci & Med Engn, State Key Lab Mol Elect, Nanjing 210096, Peoples R China

[3] Jiangsu Ctr Dis Prevent & Control, Nanjing 210009, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2006年 / 27卷 / 08期

关键词：

immunoassay; chemiluminescence; magnetic beads; Escherichia coli;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A high sensitive chemiluminescent magnetic enzyme-linked immunoassay method was established for Escherichia coli O157:H7 determination. The bacterium antibody was labeled by alkaline phosphatase ALP) that catalyzed the decomposing of substrate 3-(2-spiroadamantane)-4-methoxy-4 (3-phosphoryloxy phenyl-1,2-dioxetane(AMPPD) to give the light emission The sensitivity of the method is 8.5 x 10(4) Cell/mL with a linear range of 1.0 x 10(5)-5.0 x 10(7) Cell/mL. The intra- and. inter-assay CVs are 14.8% and 20.0% in pork samples, respectively. The correlation coefficient of present and the standard plate counting method is 0.981. The experiments with the spiked samples show that this method has great potential to be applied to detecting the concentration of Escherichia coli O157: H7 in a variety of samples.

引用

页码：1453 / 1455

页数：3

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