Cytochrome P450 2D6 based electrochemical sensor for the determination of codeine

被引：21

作者：

Asturias-Arribas, Laura ^{[1
]}

Asuncion Alonso-Lomillo, M. ^{[1
]}

Dominguez-Renedo, Olga ^{[1
]}

Julia Arcos-Martinez, M. ^{[1
]}

机构：

[1] Univ Burgos, Fac Sci, Dept Analyt Chem, Burgos 09001, Spain

来源：

TALANTA | 2014年 / 129卷

关键词：

Codeine; Cytochrome P450 2D6; Screen-printed electrode; Biosensor; VOLTAMMETRIC DETERMINATION; ALUMINUM ELECTRODE; CARBON ELECTRODES; ASCORBIC-ACID; MORPHINE; BIOSENSORS; DRUGS; PHARMACEUTICALS; PARACETAMOL; METABOLITES;

D O I：

10.1016/j.talanta.2014.05.053

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Considering the enzymatic activity of the cytochrome P450 2D6 on substrates such as codeine, the current paper includes the development of an enzymatic biosensor for detection of this drug. Home-made screen-printed electrodes were used as electrochemical transducers of the biosensor, in which the enzyme was covalently attached to the carbon surface of the working electrode, this type of modification being the most suitable for the immobilization of the biological element. Chronoamperometric measurements were carried out under optimum conditions of pH and working potential, pH 7 and +200 mV vs. screen-printed Ag/AgCl electrode, giving a reduction signal related to the concentration of codeine in solution. Consecutive additions of a solution of codeine were performed to obtain calibration curves in order to validate the electrochemical method in terms of precision and calculate its capability of detection. These biosensors were used for the determination of codeine in urine and commercial pharmaceutical samples. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：315 / 319

页数：5

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