Identification of a common conserved neutralizing linear B-cell epitope in the VP3 protein of waterfowl parvoviruses

被引：7

作者：

Lian, Caiyu ^{[1
,2
,3
]}

Zhang, Ruihua ^{[1
,2
,3
]}

Lan, Jingjing ^{[1
,2
,3
]}

Yang, Yupeng ^{[1
,2
]}

Li, Hanqing ^{[1
,2
]}

Sui, Nana ^{[1
,2
,3
]}

Xie, Zhijing ^{[1
,2
,3
]}

Jiang, Shijin ^{[1
,2
,3
]}

机构：

[1] Shandong Agr Univ, Coll Vet Med, Dept Prevent Vet Med, Tai An, Shandong, Peoples R China

[2] Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An, Shandong, Peoples R China

[3] Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An, Shandong, Peoples R China

来源：

AVIAN PATHOLOGY | 2020年 / 49卷 / 04期

关键词：

Goose parvovirus; novel goose parvovirus-related virus; Muscovy duck parvovirus; monoclonal antibody; VP3; protein; epitope; MUSCOVY DUCK PARVOVIRUS; ORIGIN GOOSE PARVOVIRUS; DWARFISM SYNDROME; SHORT BEAK; PCR ASSAY; VIRUS; PATHOGENICITY; DOMESTICUS; OUTBREAK; TYPE-1;

D O I：

10.1080/03079457.2020.1746743

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Waterfowl parvoviruses (WPVs) including goose parvovirus (GPV), novel GPV-related virus (NGPV) and Muscovy duck parvovirus (MDPV) cause significant economic losses and epizootic threat to the waterfowl industries, and little is known about the B-cell epitopes of WPVs. In this study, a monoclonal antibody (mAb) 5B5 against the VP3 protein of NGPV was used to identify the possible epitope in the three kinds of WPVs. The mAb 5B5 had neutralizing activities to the three viruses, and reacted with the conserved linear B-cell epitopes of(438)LHNPPP(443)in VP3 protein of GPV, NGPV and MDPV. To the authors' best knowledge, this is the first report on identification of the common conserved neutralizing linear B-cell epitope on VP3 protein of three different WPVs, which would facilitate the development of a novel immunodiagnostic assay for rapid detection of WPV infection.

引用

页码：325 / 334

页数：10

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