Sulforaphane attenuates obesity by inhibiting adipogenesis and activating the AMPK pathway in obese mice

被引:133
作者
Choi, Kyeong-Mi [1 ,2 ]
Lee, Youn-Sun [1 ,2 ]
Kim, Wonkyun [1 ,2 ]
Kim, Seung Jung [3 ]
Shin, Kyong-Oh [1 ,2 ]
Yu, Ji-Yeon [4 ]
Lee, Mi Kyeong [1 ,2 ]
Lee, Yong-Moon [1 ,2 ]
Hong, Jin Tae [1 ,2 ]
Yun, Yeo-Pyo [1 ,2 ]
Yoo, Hwan-Soo [1 ,2 ]
机构
[1] Chungbuk Natl Univ, Coll Pharm, Cheonu 361763, South Korea
[2] Chungbuk Natl Univ, Ctr Innovat Canc Therapeut, Cheonu 361763, South Korea
[3] Chungbuk Natl Univ, Dept Med, Cheonu 361763, South Korea
[4] Korea Res Inst Chem Technol, Lab Chem Genom, Taejon 305606, South Korea
基金
新加坡国家研究基金会;
关键词
Sulforaphane; Obesity; Adipogenesis; Lipogenesis; AMPK; Leptin; PROTEIN-KINASE CASCADE; DIET-INDUCED OBESITY; CELLULAR-ENERGY; FATTY-ACID; ADIPOCYTE; INFLAMMATION; ADIPONECTIN; METABOLISM; MINIREVIEW;
D O I
10.1016/j.jnutbio.2013.10.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Obesity is associated with metabolic disorders. Sulforaphane, an isothiocyanate, inhibits adipogenesis and the occurrence of cardiovascular disease. In this study, we investigated whether sulforaphane could prevent high-fat diet (HFD)-induced obesity in C57BL/6N mice. Mice were fed a normal diet (ND), HFD or HFD plus 0.1% sulforaphane (SFN) for 6 weeks. Food efficiency ratios and body weight were lower in HFD-SFN-fed mice than in HFD-fed mice. SFN attenuated HFD-induced visceral adiposity, adipocyte hypertrophy and fat accumulation in the liver. Serum total cholesterol and leptin, and liver triglyceride levels were lower in HFD-SFN-fed mice than in HFD-fed mice. SFN decreased the expression of peroxisome proliferator-activated receptor gamma (PPAR-gamma), CCAAT/enhancer-binding protein alpha (C/EBP alpha) and leptin in the adipose tissue of HFD-SFN mice and increased adiponectin expression. Phosphorylation of AMP-activated protein kinase alpha (AMPK alpha) and acetyl-CoA carboxylase in the adipose tissue of HFD-SFN-fed mice was elevated, and HMG-CoA reductase expression was decreased compared with HFD-fed mice. Thus, these results suggest that SFN may induce antiobesity activity by inhibiting adipogenesis through down-regulation of PPAR gamma and C/EBP alpha and by suppressing lipogenesis through activation of the AMPK pathway. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:201 / 207
页数:7
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