Evaluation of Three-Dimensional Bioprinted Human Cartilage Powder Combined with Micronized Subcutaneous Adipose Tissues for the Repair of Osteochondral Defects in Beagle Dogs

被引:6
作者
Ryu, Jina [1 ]
Brittberg, Mats [2 ,3 ]
Nam, Bomi [1 ]
Chae, Jinyeong [1 ]
Kim, Minju [1 ]
Iban, Yhan Colon [4 ]
Magneli, Martin [4 ,5 ,6 ]
Takahashi, Eiji [4 ,5 ,7 ]
Khurana, Bharti [8 ]
Bragdon, Charles R. [4 ,5 ]
机构
[1] ROKIT Healthcare Inc, Seoul 08514, South Korea
[2] Univ Gothenburg, Joint Restorat Ctr, Cartilage Res Unit, S-41345 Gothenburg, Sweden
[3] Kungsbaka Hosp, Reg Halland Orthopaed, S-43440 Kungsbacka, Sweden
[4] Massachusetts Gen Hosp, Harris Orthopaed Lab, Boston, MA 02114 USA
[5] Harvard Med Sch, Dept Orthopaed Surg, Boston, MA 02115 USA
[6] Danderyd Hosp, Karolinska Inst, Dept Clin Sci, S-18288 Stockholm, Sweden
[7] Kanazawa Med Univ, Dept Orthopaed Surg, Uchinada, Ishikawa 9200293, Japan
[8] Harvard Med Sch, Brigham & Womens Hosp, Dept Radiol, Boston, MA 02115 USA
关键词
bioink; cartilage; extracellular matrix; osteoarthritis; 3D printing; ATTRIBUTABLE ACTIVITY LIMITATION; DOCTOR-DIAGNOSED ARTHRITIS; PREVALENCE;
D O I
10.3390/ijms23052743
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cartilage lesions are difficult to repair due to low vascular distribution and may progress into osteoarthritis. Despite numerous attempts in the past, there is no proven method to regenerate hyaline cartilage. The purpose of this study was to investigate the ability to use a 3D printed biomatrix to repair a critical size femoral chondral defect using a canine weight-bearing model. The biomatrix was comprised of human costal-derived cartilage powder, micronized adipose tissue, and fibrin glue. Bilateral femoral condyle defects were treated on 12 mature beagles staged 12 weeks apart. Four groups, one control and three experimental, were used. Animals were euthanized at 32 weeks to collect samples. Significant differences between control and experimental groups were found in both regeneration pattern and tissue composition. In results, we observed that the experimental group with the treatment with cartilage powder and adipose tissue alleviated the inflammatory response. Moreover, it was found that the MOCART score was higher, and cartilage repair was more organized than in the other groups, suggesting that a combination of cartilage powder and adipose tissue has the potential to repair cartilage with a similarity to normal cartilage. Microscopically, there was a well-defined cartilage-like structure in which the mid junction below the surface layer was surrounded by a matrix composed of collagen type I, II, and proteoglycans. MRI examination revealed significant reduction of the inflammation level and progression of a cartilage-like growth in the experimental group. This canine study suggests a promising new surgical treatment for cartilage lesions.
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页数:14
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