The Anopheles gambiae salivary protein gSG6: An anopheline-specific protein with a blood-feeding role

被引：44

作者：

Lombardo, Fabrizio ^{[2
]}

Ronca, Raffaele ^{[1
]}

Rizzo, Cinzia ^{[2
]}

Mestres-Simon, Montserrat ^{[2
]}

Lanfrancotti, Alessandra ^{[2
]}

Curra, Chiara ^{[3
]}

Fiorentino, Gabriella ^{[1
]}

Bourgouin, Catherine ^{[4
]}

Ribeiro, Jose M. C. ^{[5
]}

Petrarca, Vincenzo ^{[6
]}

Ponzi, Marta ^{[3
]}

Coluzzi, Mario ^{[2
]}

Arca, Bruno ^{[1
,2
]}

机构：

[1] Univ Naples Federico II, Dipartimento Biol Strutturale & Funz, I-80126 Naples, Italy

[2] Univ Roma La Sapienza, Sez Parassitol, Dipartimento Sci Sanita Pubbl, I-00185 Rome, Italy

[3] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome, Italy

[4] Inst Pasteur, Ctr Prod & Infect Anopheles, F-75724 Paris, France

[5] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD 20852 USA

[6] Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2009年 / 39卷 / 07期

关键词：

Anopheles gambiae; gSG6; Salivary glands; Saliva; Blood feeding; ADULT FEMALE MOSQUITO; GLAND TRANSCRIPTOME; ANTIBODY-RESPONSE; ARTHROPOD SALIVA; AEDES; SIALOME; ANTIGENS; INSIGHT; CATALOG; LEISHMANIASIS;

D O I：

10.1016/j.ibmb.2009.04.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Anopheles gambiae salivary gland protein 6 (gSG6) is a small protein specifically found in the salivary glands of adult female mosquitoes. We report here the expression of a recombinant form of the protein and we show that in vivo gSG6 is expressed in distal-lateral lobes and is secreted with the saliva while the female mosquito probes for feeding. Injection of gSG6 dsRNA into adult A. gambiae females results in decreased gSG6 protein levels, increased probing time and reduced blood feeding ability. gSG6 orthologs have been found so far only in the salivary glands of Anopheles stephensi and Anopheles funestus, both members of the Cellia subgenus. We report here the gSG6 sequence from five additional anophelines, four species of the A. gambiae complex and Anopheles freeborni, a member of the subgenus Anopheles. We conclude that gSG6 plays some essential blood feeding role and was recruited in the anopheline subfamily most probably after the separation of the lineage which gave origin to Cellia and Anopheles subgenera. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：457 / 466

页数：10

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