Adrenergic signalling between rat taste receptor cells

被引:46
作者
Herness, S
Zhao, FL
Kaya, N
Lu, SG
Shen, TS
Sun, XD
机构
[1] Ohio State Univ, Coll Dent, Dept Oral Biol, Columbus, OH 43210 USA
[2] Ohio State Univ, Coll Med, Dept Neurosci, Columbus, OH 43210 USA
[3] Pharmacia Corp, Preclin Toxicol Infect Dis Safety Pharmacol, Kalamazoo, MI 49007 USA
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2002年 / 543卷 / 02期
关键词
D O I
10.1113/jphysiol.2002.020438
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In taste buds, synaptic transmission is traditionally thought to occur from taste receptor cells to the afferent nerve. This communication reports the novel observation that taste receptor cells respond to adrenergic stimulation. Noradrenaline application inhibited outward potassium currents in a dose-dependent manner. This inhibition was mimicked by the beta agonist isoproterenol and blocked by the beta antagonist propranolol. The alpha agonists clonidine and phenylephrine both inhibited the potassium currents and elevated intracellular calcium levels. Inwardly rectifying potassium currents were unaffected by adrenergic stimulation. Experiments using the RT-PCR technique demonstrate that lingual epithelium expresses multiple alpha (alpha1a, alpha1b, alpha1c, alpha1d, alpha2a, alpha2b, alpha2c) and beta (beta1, beta2) subtypes of adrenergic receptors, and immunocytochemistry localized noradrenaline to a subset of taste receptor cells. Collectively, these data imply strongly that adrenergic transmission within the taste bud may play a paracrine role in taste physiology.
引用
收藏
页码:601 / 614
页数:14
相关论文
共 43 条
[1]   Responses to glutamate in rat taste cells [J].
Bigiani, A ;
Delay, RJ ;
Chaudhari, N ;
Kinnamon, SC ;
Roper, SD .
JOURNAL OF NEUROPHYSIOLOGY, 1997, 77 (06) :3048-3059
[2]  
Caicedo A, 2000, J COMP NEUROL, V417, P315
[3]  
Caicedo A, 2000, J NEUROSCI, V20, P7978
[4]   INFLUENCE OF INNERVATION ON THE LEVELS OF NORADRENALINE AND SEROTONIN IN THE CIRCUMVALLATE PAPILLA OF THE RAT [J].
CANO, J ;
LOBERA, B ;
RODRIGUEZECHANDIA, EL .
JOURNAL OF NEUROBIOLOGY, 1982, 13 (01) :1-7
[5]   EFFECT OF TESTOSTERONE ON SEROTONIN AND NORADRENALINE CONCENTRATIONS AND TASTE BUD CELL NUMBER OF RAT CIRCUMVALLATE PAPILLA [J].
CANO, J ;
MACHADO, A ;
ROZA, C ;
RODRIGUEZECHANDIA, E .
CHEMICAL SENSES, 1982, 7 (02) :109-116
[6]   A metabotropic glutamate receptor variant functions as a taste receptor [J].
Chaudhari, N ;
Landin, MA ;
Roper, SD .
NATURE NEUROSCIENCE, 2000, 3 (02) :113-119
[7]  
Chaudhari N, 1996, J NEUROSCI, V16, P3817
[8]   Neural responses to bitter compounds in rats [J].
Dahl, M ;
Erickson, RP ;
Simon, SA .
BRAIN RESEARCH, 1997, 756 (1-2) :22-34
[9]  
Finger TE, 2000, NEUROBIOLOGY TASTE S, P287
[10]   VARIOUS RAT ADULT TISSUES EXPRESS ONLY ONE MAJOR MESSENGER-RNA SPECIES FROM THE GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE MULTIGENIC FAMILY [J].
FORT, P ;
MARTY, L ;
PIECHACZYK, M ;
ELSABROUTY, S ;
DANI, C ;
JEANTEUR, P ;
BLANCHARD, JM .
NUCLEIC ACIDS RESEARCH, 1985, 13 (05) :1431-1442