Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde

被引：33
|
作者：
Korzhevskii, D. E. [1 ]
Sukhorukova, E. G. [1 ]
Kirik, O. V. [1 ]
Grigorev, I. P. [1 ]
机构：
[1] Russian Acad Med Sci, Inst Expt Med, Dept Gen & Specif Morphol, Lab Funct Morphol Cent & Peripheral Nervous Syst, St Petersburg P-22, Russia
来源：
EUROPEAN JOURNAL OF HISTOCHEMISTRY | 2015年 / 59卷 / 03期
基金：
俄罗斯科学基金会;
关键词：
Fixation; zinc-ethanol-formalin; immunohistochemistry; human brain; FORMALIN FIXATION; TISSUE FIXATION; PRESERVATION; PROTEIN; DNA; MORPHOLOGY; MICROGLIA; NEURONS; RNA;
D O I：
10.4081/ejh.2015.2530
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Tissue fixation is critical for immunohistochemistry. Recently, we developed a zinc-ethanol-formalin fixative (ZEF). and the present study was aimed to assess the applicability of the ZEF for the human brain histology and immunohistochemistry and to evaluate the detectability of different antigens in the human brain fixed with ZEF. In total. 11 antigens were tested. including NeuN. neuron specific enolase. GFAP, Iba-1, calbindin, calretinin, choline acetyltransferase, glutamic acid decarboxylase (GAD65), tyrosine hydroxylase, synaptophysin, and alpha-tubulin. The obtained data show that: i) the ZEF has potential for use in general histological practice, where detailed characterization of human brain morphology is needed; ii) the antigens tested are well in the human brain specimens fixed in the ZEF.
引用
收藏
页码：233 / 237
页数：5
相关论文
共 13 条
  • [1] Detection of neuronal and glial antigens after decalcification in formic acid solution and fixation in zinc-ethanol-formaldehyde
    Kolos E.A.
    Korzhevskii D.E.
    Neuroscience and Behavioral Physiology, 2014, 44 (7) : 790 - 792
  • [2] Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens
    Arnold, MM
    Srivastava, S
    Fredenburgh, J
    Stockard, CR
    Myers, RB
    Grizzle, WE
    BIOTECHNIC & HISTOCHEMISTRY, 1996, 71 (05) : 224 - 230
  • [3] Combination of specific histochemical staining of eosinophils and mast cells with immunohistochemical demonstration of neural antigens
    Duffy, JP
    Smith, PJ
    Darnton, SJ
    Crocker, J
    Matthews, HR
    JOURNAL OF HISTOTECHNOLOGY, 1998, 21 (01) : 29 - 31
  • [4] IMMUNOHISTOCHEMICAL DEMONSTRATION OF PROTEIN-KINASE-C ISOZYMES IN HUMAN BRAIN-TUMORS
    TODO, T
    SHITARA, N
    NAKAMURA, H
    TAKAKURA, K
    IKEDA, K
    NEUROSURGERY, 1991, 29 (03) : 399 - 404
  • [5] IMMUNOHISTOCHEMICAL DEMONSTRATION OF DNA POLYMERASE-ALPHA IN HUMAN BRAIN-TUMOR CELLS
    KUNISHIO, K
    MISHIMA, N
    MATSUHISA, T
    TSUNO, K
    MATSUMI, N
    SATOH, T
    MATSUMOTO, K
    FURUTA, T
    NISHIMOTO, A
    SHIRAISHI, T
    JOURNAL OF NEUROSURGERY, 1990, 72 (02) : 268 - 272
  • [6] APPLICATION OF LECTIN AND LYMPHOCYTE-B-SPECIFIC MONOCLONAL-ANTIBODIES FOR THE DEMONSTRATION OF HUMAN MICROGLIA IN FORMALIN-FIXED, PARAFFIN-EMBEDDED BRAIN-TISSUE
    SASAKI, A
    NAKANISHI, Y
    NAKAZATO, Y
    YAMAGUCHI, H
    VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY, 1991, 419 (04) : 291 - 299
  • [7] IMMUNOHISTOCHEMICAL DEMONSTRATION OF L-ORNITHINE DECARBOXYLASE ENZYME PROTEIN IN DIFFERENT AREAS OF THE DEVELOPING HUMAN BRAIN
    MULLER, M
    PAJUNEN, AEI
    JARVINEN, M
    POEGGEL, G
    BERNSTEIN, HG
    DEVELOPMENTAL NEUROSCIENCE, 1993, 15 (02) : 87 - 93
  • [8] Immunohistochemical demonstration of the zinc metalloprotease insulin-degrading enzyme in normal and malignant human breast: Correlation with tissue insulin levels
    Radulescu, Razvan T.
    Hufnagel, Carla
    Luppa, Peter
    Hellebrand, Heide
    Kuo, Wen-Liang
    Rosner, Marsha Rich
    Harbeck, Nadia
    Giersig, Cecylia
    Meindl, Alfons
    Schmitt, Manfred
    Weirich, Gregor
    INTERNATIONAL JOURNAL OF ONCOLOGY, 2007, 30 (01) : 73 - 80
  • [9] Immunohistochemical demonstration of the amphetamine derivatives 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in human post-mortem brain tissues and the pituitary gland
    De Letter, EA
    Espeel, MFA
    Craeymeersch, MEC
    Lambert, WE
    Clauwaert, KM
    Dams, R
    Mortier, KA
    Piette, MHA
    INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2003, 117 (01) : 2 - 9
  • [10] Immunohistochemical demonstration of the amphetamine derivatives 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in human post-mortem brain tissues and the pituitary gland
    Els A. De Letter
    Marc F. A. Espeel
    Marijke E. C. Craeymeersch
    Willy E. Lambert
    Karine M. Clauwaert
    Riet Dams
    Kjell A. Mortier
    Michel H. A. Piette
    International Journal of Legal Medicine, 2003, 117 : 2 - 9
12