Characterization of an Insoluble and Soluble Form of Melanin Produced by Streptomyces cavourensis SV 21, a Sea Cucumber Associated Bacterium

被引:24
作者
Wibowo, Joko Tri [1 ,2 ]
Kellermann, Matthias Y. [1 ]
Petersen, Lars-Erik [1 ,7 ]
Alfiansah, Yustian R. [3 ,4 ]
Lattyak, Colleen [5 ]
Schupp, Peter J. [1 ,6 ]
机构
[1] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm ICBM, Schleusenstr 1, D-26382 Wilhelmshaven, Germany
[2] Natl Res & Innovat Agcy BRIN, Biotechnol Res Ctr, Jl Raya Bogor KM 46, Cibinong 16911, Indonesia
[3] Natl Res & Innovat Agcy BRIN, Res Ctr Oceanog, Jl Pasir Putih 1, Jakarta Utara 14430, Indonesia
[4] Helmholtz Ctr Polar & Marine Res, Ctr Aquaculture Res ZAF, AWI, Handelshafen 12, D-27570 Bremerhaven, Germany
[5] DLR Inst Networked Energy Syst, D-26129 Oldenburg, Germany
[6] Univ Oldenburg HIFMB, Helmholtz Inst Funct Marine Biodivers, Ammerlander Heerstr 231, D-26129 Oldenburg, Germany
[7] Weizmann Inst Sci, Dept Plant & Environm Sci, IL-7610001 Rehovot, Israel
关键词
melanin pigment; Streptomyces; water soluble; quorum sensing inhibition; antioxidant; PIGMENT; PURIFICATION; RADIATION; OXIDATION; CELLS;
D O I
10.3390/md20010054
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Melanin is a widely distributed and striking dark-colored pigment produced by countless living organisms. Although a wide range of bioactivities have been recognized, there are still major constraints in using melanin for biotechnological applications such as its fragmentary known chemical structure and its insolubility in inorganic and organic solvents. In this study, a bacterial culture of Streptomyces cavourensis SV 21 produced two distinct forms of melanin: (1) a particulate, insoluble form as well as (2) a rarely observed water-soluble form. The here presented novel, acid-free purification protocol of purified particulate melanin (PPM) and purified dissolved melanin (PDM) represents the basis for an in-depth comparison of their physicochemical and biological properties, which were compared to the traditional acid-based precipitation of melanin (AM) and to a synthetic melanin standard (SM). Our data show that the differences in solubility between PDM and PPM in aqueous solutions may be a result of different adjoining cation species, since the soluble PDM polymer is largely composed of Mg2+ ions and the insoluble PPM is dominated by Ca2+ ions. Furthermore, AM shared most properties with SM, which is likely attributed to a similar, acid-based production protocol. The here presented gentler approach of purifying melanin facilitates a new perspective of an intact form of soluble and insoluble melanin that is less chemical altered and thus closer to its original biological form.
引用
收藏
页数:20
相关论文
共 52 条
[21]   Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus [J].
Kimes, Nikole E. ;
Grim, Christopher J. ;
Johnson, Wesley R. ;
Hasan, Nur A. ;
Tall, Ben D. ;
Kothary, Mahendra H. ;
Kiss, Hajnalka ;
Munk, A. Christine ;
Tapia, Roxanne ;
Green, Lance ;
Detter, Chris ;
Bruce, David C. ;
Brettin, Thomas S. ;
Colwell, Rita R. ;
Morris, Pamela J. .
ISME JOURNAL, 2012, 6 (04) :835-846
[22]   Characterization of water-soluble dark-brown pigment from Antarctic bacterium, Lysobacter oligotrophicus [J].
Kimura, Tomomi ;
Fukuda, Wakao ;
Sanada, Tomoe ;
Imanaka, Tadayuki .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2015, 120 (01) :58-61
[23]   Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilmforming multi drug resistant Staphylococcus aureus [J].
Kiran, George Seghal ;
Jackson, Stephen A. ;
Priyadharsini, Sethu ;
Dobson, Alan D. W. ;
Selvin, Joseph .
SCIENTIFIC REPORTS, 2017, 7
[24]   Data on the characterization of non-cytotoxic pyomelanin produced by marine Pseudomonas stutzeri BTCZ10 with cosmetological importance [J].
Kurian, Noble K. ;
Bhat, Sarita G. .
DATA IN BRIEF, 2018, 18 :1889-1894
[25]  
KUZNETSOV V D, 1984, Mikrobiologiya, V53, P251
[26]   Purification, characterisation and biological activity of melanin from Streptomyces sp. [J].
Li, Chaofeng ;
Ji, Chenming ;
Tang, Boping .
FEMS MICROBIOLOGY LETTERS, 2018, 365 (19) :1-8
[27]   A Bacillus thuringiensis host strain with high melanin production for preparation of light-stable biopesticides [J].
Liu, Fengxia ;
Yang, Wenjun ;
Ruan, Lifang ;
Sun, Ming .
ANNALS OF MICROBIOLOGY, 2013, 63 (03) :1131-1135
[28]   Ion-exchange and adsorption of Fe(III) by Sepia melanin [J].
Liu, Y ;
Hong, L ;
Kempf, VR ;
Wakamatsu, K ;
Ito, S ;
Simon, JD .
PIGMENT CELL RESEARCH, 2004, 17 (03) :262-269
[29]   Production and Cytotoxicity of Extracellular Insoluble and Droplets of Soluble Melanin by Streptomyces lusitanus DMZ-3 [J].
Madhusudhan, D. N. ;
Mazhari, Bi Bi Zainab ;
Dastager, Syed G. ;
Agsar, Dayanand .
BIOMED RESEARCH INTERNATIONAL, 2014, 2014
[30]   Isolation and characterization of biologically active melanin from Actinoalloteichus sp MA-32 [J].
Manivasagan, Panchanathan ;
Venkatesan, Jayachandran ;
Senthilkumar, Kalimuthu ;
Sivakumar, Kannan ;
Kim, Se-Kwon .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 58 :263-274